PROCR Human, Sf9
Sf9, Baculovirus cells.
Protein C Receptor, CD201, APC Receptor, EPCR, Centrocyclin, CCD41, CCCA.
Greater than 95.0% as determined by SDS-PAGE.
Description
PROCR Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 435 amino acids (18-210a.a.) and having a molecular mass of 49.3kDa (Molecular size on SDS-PAGE will appear at approximately 40-57kDa). PROCR is expressed with a 242 amino acids hIgG-His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Protein C Receptor, CD201, APC Receptor, EPCR, Centrocyclin, CCD41, CCCA.
Sf9, Baculovirus cells.
ADPSQDASDG LQRLHMLQIS YFRDPYHVWY QGNASLGGHL THVLEGPDTN TTIIQLQPLQ EPESWARTQS GLQSYLLQFH GLVRLVHQER TLAFPLTIRC FLGCELPPEG SRAHVFFEVA VNGSSFVSFR PERALWQADT QVTSGVVTFT LQQLNAYNRT RYELREFLED TCVQYVQKHI SAENTKGSQT SRSYTSLEPK SCDKTHTCPP CPAPELLGGP SVFLFPPKPK DTLMISRTPE VTCVVVDVSH EDPEVKFNWY VDGVEVHNAK TKPREEQYNS TYRVVSVLTV LHQDWLNGKE YKCKVSNKAL PAPIEKTISK AKGQPREPQV YTLPPSRDEL TKNQVSLTCL VKGFYPSDIA VEWESNGQPE NNYKTTPPVL DSDGSFFLYS KLTVDKSRWQ QGNVFSCSVM HEALHNHYTQ KSLSLSPGKH HHHHH.
Product Science Overview
Protein-C receptor, also known as endothelial protein C receptor (EPCR), is a critical component in the protein C anticoagulant pathway. This receptor plays a significant role in regulating blood coagulation, inflammation, and cell death. The recombinant form of this receptor, expressed in Sf9 cells, has been extensively studied for its therapeutic potential and biological functions.
The Sf9 cell line, derived from the fall armyworm Spodoptera frugiperda, is widely used for the expression of recombinant proteins. The baculovirus expression system in Sf9 cells is particularly advantageous for producing high levels of functional proteins, including G-protein-coupled receptors (GPCRs) and other complex proteins . This system allows for the co-expression of multiple proteins, facilitating the study of protein interactions and functions.
The protein-C receptor is a transmembrane glycoprotein that binds activated protein C (APC). APC is a serine protease with potent anticoagulant and anti-inflammatory properties . The binding of APC to EPCR enhances its anticoagulant activity by promoting the inactivation of factors Va and VIIIa, which are essential for blood clot formation. Additionally, the APC-EPCR complex can activate protease-activated receptor 1 (PAR-1), leading to various cellular responses, including anti-apoptotic and anti-inflammatory effects .
Recombinant EPCR expressed in Sf9 cells has been investigated for its potential therapeutic applications. The ability to produce large quantities of functional EPCR allows for detailed studies on its role in various diseases, including sepsis, thrombosis, and inflammatory disorders. The recombinant receptor can be used to develop novel therapeutic agents that target the protein C pathway, offering new treatment options for patients with coagulation and inflammatory disorders.
