PROCR Human

Protein-c Receptor Human Recombinant
Cat. No.
BT8955
Source
Escherichia Coli.
Synonyms

Protein C Receptor, CD201, APC Receptor, EPCR, Centrocyclin, CCD41, CCCA

Appearance
Sterile Filtered colorless solution.
Purity
Greater than 80.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PROCR Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 216 amino acids (18-210) and having a molecular mass of 24.4 kDa.
PROCR is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
The protein encoded by the PROCR gene serves as a receptor for activated protein C (APC), a serine protease with a crucial role in the blood coagulation cascade. This receptor, known as Protein-c Receptor (PROCR), is a type I membrane protein that undergoes N-glycosylation. It enhances the activation of protein C, thereby playing a role in regulating coagulation. Mutations in the PROCR gene have been associated with an increased risk of venous thromboembolism and myocardial infarction, as well as complications during pregnancy such as late fetal loss. Additionally, PROCR may be implicated in malarial infection and has been linked to cancer.
Description
This product consists of a recombinant human PROCR protein produced in E.coli. It is a single, non-glycosylated polypeptide chain comprising 216 amino acids (residues 18-210) with a molecular weight of 24.4 kDa. The protein includes a 23 amino acid His-tag fused at the N-terminus to facilitate purification, which is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless, and sterile-filtered solution.
Formulation
This PROCR solution is provided at a concentration of 1 mg/ml and is formulated in a buffer containing 20mM Tris-HCl (pH 8.0), 10% glycerol, and 0.4M Urea.
Stability
For short-term storage (up to 2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability during frozen storage, consider adding a carrier protein such as HSA or BSA (0.1%). Repeated freezing and thawing of the product should be avoided.
Purity
The purity of this product is greater than 80.0% as determined by SDS-PAGE analysis.
Synonyms

Protein C Receptor, CD201, APC Receptor, EPCR, Centrocyclin, CCD41, CCCA

Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSSQDASDG LQRLHMLQIS YFRDPYHVWY QGNASLGGHL THVLEGPDTN TTIIQLQPLQ EPESWARTQS GLQSYLLQFH GLVRLVHQER TLAFPLTIRC FLGCELPPEG SRAHVFFEVA VNGSSFVSFR PERALWQADT QVTSGVVTFT LQQLNAYNRT RYELREFLED TCVQYVQKHI SAENTKGSQT SRSYTS.

Product Science Overview

Introduction

The Protein C receptor, also known as the Endothelial Protein C Receptor (EPCR), plays a crucial role in the anticoagulation pathway. It is primarily found on the surface of endothelial cells, which line the interior of blood vessels. The recombinant form of this receptor is produced using genetic engineering techniques to study its structure, function, and therapeutic potential.

Structure and Function

EPCR is a transmembrane glycoprotein that binds Protein C, a vitamin K-dependent serine protease zymogen. Upon binding to EPCR, Protein C is activated by the thrombin-thrombomodulin complex to form Activated Protein C (APC). APC plays a significant role in regulating blood coagulation, inflammation, and cell death .

The structure of EPCR includes an extracellular domain that binds Protein C, a single transmembrane domain, and a short cytoplasmic tail. The extracellular domain is crucial for its interaction with Protein C and other ligands .

Biological Significance

EPCR enhances the activation of Protein C by presenting it to the thrombin-thrombomodulin complex on the endothelial cell surface. Activated Protein C (APC) then exerts its anticoagulant effects by proteolytically inactivating Factors Va and VIIIa, which are essential for blood clot formation .

In addition to its role in anticoagulation, EPCR has been implicated in various cellular processes, including:

  • Anti-inflammatory effects: APC-EPCR interaction reduces the production of pro-inflammatory cytokines.
  • Cytoprotective effects: APC signaling through EPCR can protect endothelial cells from apoptosis.
  • Barrier function: EPCR-APC interaction helps maintain the integrity of the endothelial barrier, preventing vascular leakage .
Recombinant EPCR

Recombinant EPCR is produced using recombinant DNA technology, where the gene encoding EPCR is inserted into an expression vector and introduced into host cells (e.g., bacteria, yeast, or mammalian cells). These host cells then produce the EPCR protein, which can be purified for research and therapeutic purposes .

Recombinant EPCR is used in various studies to understand its structure-function relationship, its role in disease, and its potential as a therapeutic target. For example, recombinant EPCR has been used to study its interaction with Protein C and other ligands, as well as its effects on endothelial cell function .

Therapeutic Potential

Given its role in regulating coagulation and inflammation, EPCR is a potential therapeutic target for various conditions, including:

  • Sepsis: Recombinant human Activated Protein C (rhAPC) has been used to treat severe sepsis by reducing inflammation and improving survival rates .
  • Thrombosis: EPCR and its ligands are being explored as potential treatments for thrombotic disorders by modulating the anticoagulant pathway .
  • Inflammatory diseases: The anti-inflammatory properties of the EPCR-APC interaction make it a potential target for treating inflammatory diseases .

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