PRKCI Human
HEK293 Cells.
DXS1179E, nPKC-iota, PKCI, aPKC-lambda/iota, PRKC-lambda/iota, PRKCL, Protein kinase C iota type, Protein Kinase C iota, Atypical protein kinase C-lambda/iota, PRKCI.
Greater than 80.0% as determined by SDS-PAGE.
Description
PRKCI Human Recombinant produced in HEK cells is a single, glycosylated, polypeptide chain (1-596 a.a) containing a total of 602 amino acids, having a molecular mass of 69kDa.
PRKCI is fused to a 6 amino acid His-tag at C-terminus,and is purified by proprietary chromatographic techniques.
Product Specs
PRKCI, also known as AKAPKC iota, belongs to the serine/threonine protein kinase family and plays a crucial role in regulating various cellular processes, including hormone secretion, cell growth and differentiation, and gene expression. It is essential for cell polarization and the development of epithelial tight junctions. This kinase is calcium-independent and relies on phospholipids for its activity. PRKCI is also involved in activating multiple signaling pathways, such as Ras, NF-kappa-B, and c-Src pathways.
Recombinant human PRKCI, expressed in HEK cells, is a single, glycosylated polypeptide chain encompassing amino acids 1 to 596. With a 6-amino acid His-tag fused at the C-terminus, the protein has a total of 602 amino acids and a molecular weight of 69 kDa. The purification process involves proprietary chromatographic techniques.
The PRKCI solution is provided at a concentration of 0.5 mg/ml in a buffer consisting of 10% glycerol and Phosphate-Buffered Saline (pH 7.4).
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein such as HSA or BSA (0.1%) is advisable for long-term storage. Repeated freezing and thawing should be avoided.
The purity of the protein is determined to be greater than 80.0% by SDS-PAGE analysis.
DXS1179E, nPKC-iota, PKCI, aPKC-lambda/iota, PRKC-lambda/iota, PRKCL, Protein kinase C iota type, Protein Kinase C iota, Atypical protein kinase C-lambda/iota, PRKCI.
HEK293 Cells.
MPTQRDSSTM SHTVAGGGSG DHSHQVRVKA YYRGDIMITH FEPSISFEGL CNEVRDMCSF DNEQLFTMKW IDEEGDPCTV SSQLELEEAF RLYELNKDSE LLIHVFPCVP ERPGMPCPGE DKSIYRRGAR RWRKLYCANG HTFQAKRFNR RAHCAICTDR IWGLGRQGYK CINCKLLVHK KCHKLVTIEC GRHSLPQEPV MPMDQSSMHS DHAQTVIPYN PSSHESLDQV GEEKEAMNTR ESGKASSSLG LQDFDLLRVI GRGSYAKVLL VRLKKTDRIY AMKVVKKELV NDDEDIDWVQ TEKHVFEQAS NHPFLVGLHS CFQTESRLFF VIEYVNGGDL MFHMQRQRKL PEEHARFYSA EISLALNYLH ERGIIYRDLK LDNVLLDSEG HIKLTDYGMC KEGLRPGDTT STFCGTPNYI APEILRGEDY GFSVDWWALG VLMFEMMAGR SPFDIVGSSD NPDQNTEDYL FQVILEKQIR IPRSLSVKAA SVLKSFLNKD PKERLGCHPQ TGFADIQGHP FFRNVDWDMM EQKQVVPPFK PNISGEFGLD NFDSQFTNEP VQLTPDDDDI VRKIDQSEFE GFEYINPLLM SAEECVHHHH HH
Product Science Overview
Protein Kinase C iota (PKCι), also known as atypical protein kinase C-lambda/iota (aPKC-λ/ι) and PRKCI, is a member of the protein kinase C (PKC) family. This family of serine/threonine kinases plays a crucial role in various cellular processes, including cell proliferation, differentiation, and apoptosis. PKCι is classified as an atypical PKC due to its unique regulatory properties and structural features.
PKCι is a cytoplasmic, membrane, and nuclear protein that belongs to the AGC (protein kinase A, G, and C) superfamily. It contains several distinct domains:
- AGC-kinase C-terminal domain
- OPR domain
- Phorbol-ester/DAG-type zinc finger
- Protein kinase domain
These domains enable PKCι to interact with various substrates and regulatory proteins, facilitating its role in multiple signaling pathways. Unlike classical PKCs, PKCι is calcium-independent and phospholipid-dependent, making it unique in its activation and function.
PKCι is involved in several critical cellular processes:
- Cell Polarization: PKCι plays a vital role in establishing and maintaining cell polarity, which is essential for proper cell function and tissue organization.
- Epithelial Tight Junctions: It contributes to the formation and maintenance of tight junctions in epithelial cells, which are crucial for barrier function and tissue integrity.
- Signaling Pathways: PKCι is implicated in the activation of several signaling pathways, including Ras, c-Src, and NF-kappa-B pathways. These pathways are involved in cell growth, survival, and differentiation.
- Apoptosis: PKCι functions in both pro- and anti-apoptotic pathways, highlighting its complex role in cell survival and death.
- Microtubule Dynamics: Through its interaction with RAB2A and GAPDH, PKCι influences microtubule dynamics and is recruited to vesicular tubular clusters (VTCs), which are essential for intracellular transport .
PKCι has been shown to play a pivotal role in cancer development and progression. Its overexpression is associated with poor prognosis in various cancers, including lung adenocarcinoma. PKCι’s involvement in the Hippo/YAP pathway, which is commonly disrupted in cancers, further underscores its significance in tumorigenesis .
Recombinant human PKCι is produced using baculovirus expression systems in Sf9 insect cells. The recombinant protein is typically tagged with a GST (glutathione S-transferase) tag to facilitate purification and detection. It is supplied in a sterile buffer solution and should be stored at –70°C to maintain stability and activity .
