PRDX6 Human
Peroxiredoxin-6 Human Recombinant
Cat. No.
BT25333
Source
Escherichia Coli.
Synonyms
Peroxiredoxin-6, Antioxidant protein 2, 1-Cys peroxiredoxin, Acidic calcium-independent phospholipase A2, Non-selenium glutathione peroxidase, 24 kDa protein, Liver 2D page spot 40, Red blood cells page spot 12, 1-Cys PRX, aiPLA2, NSGPx, PRDX6, AOP2, KIAA0106, PRX, p29, 1-Cys, MGC46173.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
Peroxiredoxin- 6 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 244 amino acids (1-224 a.a.) and having a molecular mass of 27.1kDa.
The Peroxiredoxin-6 is purified by proprietary chromatographic techniques.
The Peroxiredoxin-6 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Peroxiredoxin 6 (PRDX6) is a member of the thiol-specific antioxidant protein family. This bifunctional enzyme, possessing two distinct active sites, plays a crucial role in cellular redox regulation. PRDX6 exhibits the ability to reduce hydrogen peroxide, short chain organic, fatty acid, and phospholipid hydroperoxides. Its functions extend to the regulation of phospholipid turnover and protection against oxidative damage. PRDX6 has been shown to alleviate oxidative stress and mitigate TGF-beta-induced abnormalities in human trabecular meshwork cells. Additionally, it is essential for maintaining blood vessel integrity in skin injuries. Notably, PRDX6 exhibits pH-dependent binding affinity to phospholipids, interacting with reduced phospholipids at acidic pH and oxidized phospholipids at cytosolic pH, suggesting its role in repairing peroxidized cell membranes. Hydrogen peroxide-induced hyperoxidation of PRDX6 can trigger cell cycle arrest at the G2/M transition through the up-regulation of iPLA2 activity. Overexpression of PRDX6 has been implicated in oligodendroglioma.
Description
Recombinant human Peroxiredoxin-6, expressed in E. coli, is a non-glycosylated polypeptide chain containing 244 amino acids (including a 20 amino acid His tag at the N-terminus). This protein, with a molecular weight of 27.1 kDa, is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The Peroxiredoxin-6 solution is supplied in a buffer consisting of 20mM Tris-HCl (pH 8.0) and 20% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Repeated freezing and thawing of the product should be avoided.
Purity
The purity of this product is greater than 95.0%, as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of this enzyme preparation is typically between 95 and 120 pmole/min/µg. This was determined by measuring the residual peroxide concentration following a 20-minute incubation of PRDX6 with peroxide at room temperature. Specific activity, in this context, refers to the quantity of hydroperoxide reduced by 1 µg of enzyme within 1 minute at a temperature of 25°C.
Synonyms
Peroxiredoxin-6, Antioxidant protein 2, 1-Cys peroxiredoxin, Acidic calcium-independent phospholipase A2, Non-selenium glutathione peroxidase, 24 kDa protein, Liver 2D page spot 40, Red blood cells page spot 12, 1-Cys PRX, aiPLA2, NSGPx, PRDX6, AOP2, KIAA0106, PRX, p29, 1-Cys, MGC46173.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MPGGLLLGDV APNFEANTTV GRIRFHDFLG DSWGILFSHP RDFTPVCTTE LGRAAKLAPE FAKRNVKLIA LSIDSVEDHL AWSKDINAYN CEEPTEKLPF PIIDDRNREL AILLGMLDPA EKDEKGMPVT ARVVFVFGPD KKLKLSILYP ATTGRNFDEI LRVVISLQLT AEKRVATPVD WKDGDSVMVL PTIPEEEAKK LFPKGVFTKE LPSGKKYLRY TPQP.
Product Science Overview
Structure and Expression
PRDX6 is encoded by the PRDX6 gene located on chromosome 1q25 . The human recombinant form of PRDX6 is typically expressed in Escherichia coli (E. coli) and purified for research and therapeutic purposes . The recombinant protein comprises 235 amino acids with a molecular mass of approximately 26.5 kDa .
Functional Roles
PRDX6 is involved in several critical cellular processes:
- Oxidative Stress Response: PRDX6 reduces hydrogen peroxide and organic hydroperoxides, protecting cells from oxidative damage .
- Lipid Metabolism: The iPLA2 activity of PRDX6 is essential for the repair of peroxidized cell membranes and the regulation of phospholipid turnover .
- Cellular Protection: PRDX6 plays a protective role against oxidative injury, particularly in the lungs where it is highly expressed .
Clinical Significance
PRDX6 has been implicated in various diseases and conditions:
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