PRDX3 Human
Peroxiredoxin-3 Human Recombinant
Cat. No.
BT25095
Source
Escherichia Coli.
Synonyms
AOP1, MER5, AOP-1, SP-22, PRO1748, MGC24293, MGC104387, PRDX3, Thioredoxin-dependent peroxide reductase mitochondrial, Peroxiredoxin-3, PRX III, Antioxidant protein 1, Protein MER5 homolog, HBC189.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PRDX3 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 195 amino acids (63-256 a.a.) and having a molecular mass of 21.5 kDa.
The PRDX3 is purified by proprietary chromatographic techniques.
The PRDX3 is purified by proprietary chromatographic techniques.
Product Specs
Introduction
Peroxiredoxin 3 (PRDX3) belongs to the peroxiredoxin family of antioxidant enzymes, known for reducing hydrogen peroxide and alkyl hydroperoxides. Found primarily in mitochondria, PRDX3 plays a crucial role in regulating cellular redox balance by acting as a first line of defense against hydrogen peroxide generated during respiration. It's a key regulator of mitochondrial H2O2 levels, which can induce apoptosis in conjunction with other apoptotic signaling molecules. Decreased levels of PRDX3 mitochondrial protein are observed in Alzheimer's Disease and Down Syndrome.
Description
Recombinant Human PRDX3, produced in E. coli, is a single, non-glycosylated polypeptide chain consisting of 195 amino acids (residues 63-256). With a molecular weight of 21.5 kDa, it is purified using proprietary chromatographic techniques.
Physical Appearance
Clear, colorless solution, sterile-filtered.
Formulation
The PRDX3 solution is supplied in 20mM Tris-HCl buffer at pH 8, with 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Minimize repeated freeze-thaw cycles.
Purity
Purity exceeds 95.0% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of the enzyme is measured at approximately 82-83 picomoles of hydroperoxide reduced per minute per microgram of enzyme. This is determined by incubating PRDX3 with peroxide at room temperature for 20 minutes and measuring the remaining peroxide. Specific activity, in this context, refers to the amount of hydroperoxide reduced by 1 microgram of enzyme in 1 minute at 25°C.
Synonyms
AOP1, MER5, AOP-1, SP-22, PRO1748, MGC24293, MGC104387, PRDX3, Thioredoxin-dependent peroxide reductase mitochondrial, Peroxiredoxin-3, PRX III, Antioxidant protein 1, Protein MER5 homolog, HBC189.
Source
Escherichia Coli.
Amino Acid Sequence
MPAVTQHAPY FKGTAVVNGE FKDLSLDDFK GKYLVLFFYP LDFTFVCPTE IVAFSDKANE FHDVNCEVVA VSVDSHFSHL AWINTPRKNG GLGHMNIALL SDLTKQISRD YGVLLEGSGL ALRGLFIIDP NGVIKHLSVN DLPVGRSVEE TLRLVKAFQY VETHGEVCPA NWTPDSPTIK PSPAASKEYF QKVNQ.
Product Science Overview
Structure and Function
Peroxiredoxin-3 is encoded by the PRDX3 gene and is highly conserved across species, including humans and mice . The enzyme functions as a thioredoxin-dependent peroxide reductase, which means it relies on thioredoxin to reduce peroxides. The primary function of PRDX3 is to protect cells from oxidative damage by reducing peroxides, thus maintaining cellular redox balance .
Expression and Purification
Recombinant human Peroxiredoxin-3 protein is typically expressed in Escherichia coli (E. coli) and purified using conventional chromatography techniques . The recombinant protein corresponds to the amino acids 63-256 of the human Peroxiredoxin-3 sequence . It is often supplied in a buffer solution containing Tris-HCl and glycerol to maintain stability and activity .
Biological Significance
Peroxiredoxin-3 is involved in several critical cellular processes:
- Redox Regulation: PRDX3 plays a significant role in regulating the redox status of cells by reducing peroxides, which are harmful byproducts of cellular metabolism .
- Protection Against Oxidative Stress: By reducing hydrogen peroxide, PRDX3 protects cells from oxidative stress, which can damage proteins, lipids, and DNA .
- Mitochondrial Function: Given its localization in the mitochondria, PRDX3 is essential for maintaining mitochondrial function and integrity .
Applications in Research
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