PRDX3 Antibody
Peroxiredoxin-3, Mouse Anti Human
Cat. No.
BT24319
Source
Synonyms
AOP1, MER5, AOP-1, SP-22, PRO1748, MGC24293, MGC104387, PRDX3,
Thioredoxin-dependent peroxide reductase mitochondrial, Peroxiredoxin-3, PRX
III, Antioxidant protein 1, Protein MER5 homolog, HBC189.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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In Stock 
Description
Product Specs
Introduction
PRDX3, a member of the peroxiredoxin family of antioxidant enzymes, plays a crucial role in reducing hydrogen peroxide and alkyl hydroperoxides. Its primary location within mitochondria highlights its involvement in regulating cellular redox balance by acting as a first line of defense against respiration-generated H2O2. PRDX3 significantly regulates mitochondrial H(2)O(2) levels, which contribute to apoptosis in conjunction with other apoptotic signaling mediators. Notably, PRDX3 mitochondrial protein expression is considerably reduced in Alzheimer's Disease and Down Syndrome.
Physical Appearance
The product is a clear and colorless solution that has undergone sterile filtration.
Formulation
The antibody is supplied at a concentration of 1 mg/ml and is formulated in a solution of PBS at pH 7.4, supplemented with 10% glycerol and 0.02% sodium azide.
Storage Procedures
The product should be stored at 4°C for short-term storage of up to 1 month. For extended storage, it is recommended to store at -20°C. Avoid repeated freeze-thaw cycles to maintain product integrity.
Stability / Shelf Life
The product remains stable for 12 months when stored at -20°C and for 1 month when stored at 4°C.
Applications
This antibody has undergone comprehensive testing in various applications, including ELISA, Western blot analysis, Flow cytometry, and ICC/IF, to ensure both its specificity and reactivity. However, it is essential to note that optimal working dilutions may vary depending on the specific application and should be determined through titration.
Synonyms
AOP1, MER5, AOP-1, SP-22, PRO1748, MGC24293, MGC104387, PRDX3,
Thioredoxin-dependent peroxide reductase mitochondrial, Peroxiredoxin-3, PRX
III, Antioxidant protein 1, Protein MER5 homolog, HBC189.
Purification Method
PRDX3 antibody was purified from mouse ascitic fluids by
protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
PAT1F8AT.
Immunogen
Anti-human PRDX3 mAb, is
derived from hybridization of mouse F0 myeloma cells with spleen cells from
BALB/c mice immunized with a recombinant human PRDX3 protein 63-256 amino
acids purified from E. coli.
Ig Subclass
Mouse IgG3 heavy chain and k light chain.
Product Science Overview
Function and Importance
PRDX3 is primarily involved in the redox regulation of cells. It helps protect radical-sensitive enzymes from oxidative damage by reducing hydrogen peroxide and organic hydroperoxides . This antioxidant function is vital for maintaining cellular homeostasis and preventing oxidative stress-related damage, which can lead to various diseases, including cancer and neurodegenerative disorders .
Mouse Anti-Human PRDX3 Antibody
The Mouse Anti-Human PRDX3 Antibody is a monoclonal antibody developed to specifically detect human PRDX3. This antibody is commonly used in various research applications, including Western blotting, immunohistochemistry, and ELISA . It is designed to bind to PRDX3, allowing researchers to study the protein’s expression, localization, and function in different biological samples.
Applications in Research
- Western Blotting: The Mouse Anti-Human PRDX3 Antibody is used to detect PRDX3 in cell lysates and tissue samples. It helps in identifying the presence and quantity of PRDX3 protein, providing insights into its role in different cellular processes .
- Immunohistochemistry: This antibody is also used to visualize PRDX3 in tissue sections. By staining tissues, researchers can observe the distribution and localization of PRDX3 within different cell types and tissues .
- ELISA: Enzyme-linked immunosorbent assay (ELISA) is another application where this antibody is used to quantify PRDX3 levels in various samples. This technique is valuable for studying changes in PRDX3 expression under different experimental conditions .
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