PPP4C Human
Protein Phosphatase 4 Catalytic subunit Human Recombinant
Cat. No.
BT1079
Source
E.coli.
Synonyms
Serine/threonine-protein phosphatase 4 catalytic subunit,
PP4, PP4C, PPH3, PPP4, PPX, PPP4C, Protein Phosphatase 4 Catalytic subunit, Protein
phosphatase X, PP-X, Pp4.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs 
In Stock 
Description
PPP4C Human Recombinant produced in E. coli is a single polypeptide
chain containing 330 amino acids (1-307) and having a molecular mass of 37.5kDa.
PPP4C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PPP4C is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
Protein Phosphatase 4 Catalytic subunit (PPP4C) is a member of the serine/threonine-protein phosphatase catalytic subunit family. This protein plays a role in dephosphorylation and is involved in regulating HDAC3. The protein phosphatase (PP) holoenzyme is a trimeric complex consisting of a regulatory subunit, a variable subunit, and a catalytic subunit. There are four main families of protein phosphatase catalytic subunits: PP1, PP2A, PP2B (calcineurin), and PP2C.
Description
PPP4C Human Recombinant protein, expressed in E. coli, is a single polypeptide chain with a molecular weight of 37.5 kDa. The protein comprises 330 amino acids, including a 23 amino acid His-tag at the N-terminus (amino acids 1-307). Purification is carried out using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The PPP4C solution is provided at a concentration of 1 mg/ml in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.4 M Urea, and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. To further enhance stability during long-term storage, the addition of a carrier protein such as HSA or BSA (0.1%) is advised. It's crucial to avoid repeated cycles of freezing and thawing.
Purity
The purity of the product is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
Serine/threonine-protein phosphatase 4 catalytic subunit,
PP4, PP4C, PPH3, PPP4, PPX, PPP4C, Protein Phosphatase 4 Catalytic subunit, Protein
phosphatase X, PP-X, Pp4.
Source
E.coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEISDL DRQIEQLRRC ELIKESEVKA LCAKAREILV EESNVQRVDS
PVTVCGDIHG QFYDLKELFR VGGDVPETNY LFMGDFVDRG FYSVETFLLL LALKVRYPDR ITLIRGNHES
RQITQVYGFY DECLRKYGSV TVWRYCTEIF DYLSLSAIID GKIFCVHGGL SPSIQTLDQI RTIDRKQEVP
HDGPMCDLLW SDPEDTTGWG VSPRGAGYLF GSDVVAQFNA ANDIDMICRA HQLVMEGYKW HFNETVLTVW
SAPNYCYRCG NVAAILELDE HLQKDFIIFE AAPQETRGIP SKKPVADYFL.
Product Science Overview
Structure and Function
The catalytic subunit of Protein Phosphatase 4 (PP4) is a protein composed of 307 amino acids with a molecular mass of approximately 35 kDa . It shares significant sequence similarity with other protein phosphatases, such as PP2A, indicating a conserved mechanism of action among these enzymes . PP4 consists of a catalytic subunit (PPP4C) that interacts with various regulatory proteins, such as PPP4R1 and PPP4R2, which determine substrate specificity and intracellular localization .
Cellular Processes
PPP4C is involved in several key cellular processes:
- Microtubule Organization: PP4 plays a role in the growth and organization of microtubules at centrosomes, which are essential for cell division and intracellular transport .
- DNA Damage Repair: PP4 is crucial for the repair of DNA double-strand breaks through homologous recombination and single-strand annealing pathways . It specifically dephosphorylates H2AX phosphorylated on Ser-140 (gamma-H2AX), a marker for DNA damage .
- Apoptosis: PP4 is involved in the regulation of apoptosis, the programmed cell death process, which is vital for maintaining cellular homeostasis and preventing cancer development .
- TNF-alpha Signaling: PP4 participates in the signaling pathway of tumor necrosis factor-alpha, a cytokine involved in inflammation and immune responses .
Clinical Significance
Research and Applications
Recombinant human PPP4C is widely used in research to study its function and regulation. By expressing and purifying the catalytic subunit in a controlled environment, researchers can investigate its interactions with other proteins and its role in cellular processes. This knowledge can contribute to the development of new therapeutic strategies for diseases associated with PP4 dysregulation.
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