PPP3R1 Human
Description
PPP3R1 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Protein Phosphatase 3 is composed of two subunits:
- Catalytic Subunit (PPP3CA): This subunit is responsible for the enzyme’s phosphatase activity. It binds to calmodulin in the presence of calcium ions, which activates the enzyme.
- Regulatory Subunit (PPP3R1): This subunit, also known as Calcineurin B, binds calcium ions and is essential for the enzyme’s activation and stability.
The alpha isozyme of the regulatory subunit (PPP3R1) is one of the isoforms that can be found in humans. It is involved in the regulation of the catalytic subunit’s activity and ensures the proper functioning of the enzyme.
Calcineurin is involved in several critical cellular pathways:
- T-Cell Activation: Calcineurin dephosphorylates the nuclear factor of activated T-cells (NFAT), allowing it to enter the nucleus and initiate the transcription of interleukin-2 (IL-2) and other cytokines, which are vital for T-cell proliferation and immune response.
- Neuronal Signaling: Calcineurin modulates synaptic plasticity and memory formation by dephosphorylating various substrates involved in neurotransmission.
- Muscle Development: Calcineurin plays a role in muscle fiber type differentiation and hypertrophy by regulating the transcription of muscle-specific genes.
The recombinant form of Protein Phosphatase 3, Regulatory Subunit, Alpha Isozyme, is produced using recombinant DNA technology. This involves cloning the gene encoding the protein into an expression vector, which is then introduced into a host cell (such as E. coli or mammalian cells). The host cells express the protein, which is subsequently purified for research or therapeutic use.
Recombinant Protein Phosphatase 3, Regulatory Subunit, Alpha Isozyme, is widely used in research to study its role in various cellular processes and diseases. It is also used in drug discovery and development, particularly in the context of immunosuppressive therapies. Calcineurin inhibitors, such as cyclosporine and tacrolimus, are commonly used to prevent organ transplant rejection by inhibiting T-cell activation.
