PPP3CA Human
Protein Phosphatase 3, Catalytic subunit, Alpha Isozyme Human Recombinant
Cat. No.
BT860
Source
Escherichia Coli.
Synonyms
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform, PPP3CA, CALNA, CAN, CALN, CCN1, CNA1, PPP2B, CALNA1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PPP3CA Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 534 amino acids (1-511 a.a.) and having a molecular mass of 60kDa. The PPP3CA is purified by proprietary chromatographic techniques.
Product Specs
Introduction
PPP3CA, also known as Calcineurin A, is a calcium/calmodulin-dependent serine/threonine protein phosphatase. It is primarily found in the brain and exhibits a limited substrate specificity. This protein plays a crucial role in activating T cells, a process that can be inhibited by drugs. PPP3CA dephosphorylates NFATc, a transcription factor, leading to its activation and subsequent translocation to the nucleus. Once in the nucleus, activated NFATc upregulates the expression of interleukin 2.
Description
Recombinant human PPP3CA, with a 23 amino acid His tag at the N-terminus, was produced in E. coli. This protein is a single, non-glycosylated polypeptide chain consisting of 534 amino acids (residues 1-511) with a molecular weight of 60 kDa. Purification of PPP3CA was achieved using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution, sterile filtered.
Formulation
The provided PPP3CA solution has a concentration of 0.25 mg/ml and is formulated in 20mM Tris-HCl buffer (pH 8.0), 0.2M NaCl, 5mM DTT, 1mM EDTA, and 20% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
The purity of PPP3CA is greater than 85.0% as assessed by SDS-PAGE.
Synonyms
Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CAM-PRP catalytic subunit, Calmodulin-dependent calcineurin A subunit alpha isoform, PPP3CA, CALNA, CAN, CALN, CCN1, CNA1, PPP2B, CALNA1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH TGSMSEPKAI DPKLSTTDRV VKAVPFPPSH RLTAKEVFDN DGKPRVDILK AHLMKEGRLE ESVALRIITE GASILRQEKN LLDIDAPVTV CGDIHGQFFD LMKLFEVGGS PANTRYLFLG DYVDRGYFSI ECVLYLWALK ILYPKTLFLL RGNHECRHLT EYFTFKQECK IKYSERVYDA CMDAFDCLPL AALMNQQFLC VHGGLSPEIN TLDDIRKLDR FKEPPAYGPM CDILWSDPLE DFGNEKTQEH FTHNTVRGCS YFYSYPAVCE FLQHNNLLSI LRAHEAQDAG YRMYRKSQTT GFPSLITIFS APNYLDVYNN KAAVLKYENN VMNIRQFNCS PHPYWLPNFM DVFTWSLPFV GEKVTEMLVN VLNICSDDEL GSEEDGFDGA TAAARKEVIR NKIRAIGKMA RVFSVLREES ESVLTLKGLT PTGMLPSGVL SGGKQTLQSA IKGFSPQHKI TSFEEAKGLD RINERMPPRR DAMPSDANLN SINKALTSET NGTDSNGSNS SNIQ.
Product Science Overview
Gene and Protein Structure
The PPP3CA gene is located on chromosome 4q24 and encodes the alpha isoform of the catalytic subunit of calcineurin . This enzyme is composed of a catalytic subunit (PPP3CA) and a regulatory subunit (PPP3R1). The catalytic subunit is responsible for the enzyme’s phosphatase activity, while the regulatory subunit binds calcium ions and calmodulin, which are essential for the enzyme’s activation .
Biological Functions
PPP3CA plays a pivotal role in various biological processes, including:
- Intracellular Signaling: It is involved in the transduction of intracellular calcium-mediated signals .
- Synaptic Vesicle Recycling: It plays a role in the recycling of synaptic vesicles, which are essential for neurotransmitter release .
- Immune Response: Calcineurin is crucial for the activation of T-cells, a type of white blood cell that plays a central role in the immune response .
Clinical Significance
Mutations in the PPP3CA gene have been associated with several diseases, including:
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