PPP2R1A Human
Description
PPP2R1A Human Recombinant produced in E.Coli is a single,
non-glycosylated polypeptide chain containing 612 amino acids (1-589 a.a) and
having a molecular mass of 67.7kDa.PPP2R1A is fused to a 23 amino acid His-tag at N-terminus
& purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
PP2A is a heterotrimeric enzyme composed of three subunits:
- Catalytic Subunit (C Subunit): This subunit is responsible for the enzyme’s phosphatase activity.
- Structural Subunit (A Subunit): Also known as the scaffolding subunit, it coordinates the assembly of the catalytic subunit and a variable regulatory subunit.
- Regulatory Subunit (B Subunit): This subunit is variable and determines the substrate specificity, localization, and activity of the PP2A holoenzyme .
The A subunit, specifically the alpha isoform (PPP2R1A), is a 65 kDa protein that serves as a scaffold for the assembly of the PP2A holoenzyme. It is composed of 15 tandemly repeated HEAT sequences, which are degenerate motifs of 39 amino acids present in various proteins .
PP2A has a broad substrate specificity and is involved in numerous cellular functions. It acts as a tumor suppressor by dephosphorylating key proteins in oncogenic signaling pathways, such as Raf, MEK, and AKT . The regulatory subunit A alpha isoform (PPP2R1A) plays a crucial role in the formation and function of the PP2A holoenzyme, influencing its enzymatic activity and substrate specificity .
The human recombinant form of the regulatory subunit A alpha (PPP2R1A) is produced using recombinant DNA technology. This involves cloning the gene encoding PPP2R1A into an expression vector, which is then introduced into a host cell (such as E. coli or yeast) for protein production. The recombinant protein is subsequently purified for use in research and therapeutic applications.
Mutations in the PPP2R1A gene have been implicated in various cancers, including ovarian clear cell carcinoma. Studies have shown that PPP2R1A functions as an oncogene in certain contexts, contributing to the pathogenesis of these cancers . Understanding the structure and function of PP2A and its regulatory subunits is crucial for developing targeted therapies for cancer and other diseases.
