PPP1R2 Human

Protein Phosphatase 1, Regulatory Subunit 2 Human Recombinant
Cat. No.
BT635
Source
Escherichia Coli.
Synonyms
PPP1R2, Protein phosphatase inhibitor 2, IPP-2, IPP2, Protein phosphatase 1, regulatory (inhibitor) subunit 2, Protein Phosphatase 1, Regulatory Subunit 2.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 85.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PPP1R2 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 189 amino acids (1-166 a.a) and having a molecular mass of 21kDa.
PPP1R2 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Protein Phosphatase 1, Regulatory Subunit 2 (PPP1R2) acts as an inhibitor of protein-phosphatase 1 by forming a heterodimer with PP1. It is classified as a member of the protein phosphatase inhibitor 2 family.
Description
Recombinant Human PPP1R2, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 189 amino acids (with amino acids 1-166 comprising the PPP1R2 sequence) and has a molecular weight of 21kDa. The recombinant protein includes a 23 amino acid His-tag fused to the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
The product is a sterile, colorless, and clear solution.
Formulation
The PPP1R2 protein is supplied in a solution at a concentration of 0.25 mg/ml. The solution also contains 20mM Tris-HCl buffer (pH 8.0), 30% glycerol, 0.15M NaCl, and 1mM DTT.
Stability
For short-term storage (2-4 weeks), the product should be stored at 4°C. For extended storage, it is recommended to store the product frozen at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). It is important to avoid repeated freeze-thaw cycles.
Purity
The purity of the protein is greater than 85%, as determined by SDS-PAGE analysis.
Synonyms
PPP1R2, Protein phosphatase inhibitor 2, IPP-2, IPP2, Protein phosphatase 1, regulatory (inhibitor) subunit 2, Protein Phosphatase 1, Regulatory Subunit 2.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAASTAS HRPIKGILKN KTSTTSSMVA SAEQPRGNVD EELSKKSQKW DEMNILATYH PADKDYGLMK IDEPSTPYHS MMGDDEDACS DTEATEAMAP DILARKLAAA EGLEPKYRIQ EQESSGEEDS DLSPEEREKK RQFEMKRKLH YNEGLNIKLA RQLISKDLH.

Product Science Overview

Structure and Function

PPP1R2 is a heat-stable protein that binds to the catalytic subunit of PP1 through an RVxF consensus sequence . This binding inactivates PP1, preventing it from dephosphorylating its substrates. The inhibition of PP1 by PPP1R2 is essential for regulating several cellular functions, including glycogen metabolism, muscle contraction, cell progression, neuronal activities, RNA splicing, mitosis, cell division, apoptosis, protein synthesis, and the regulation of membrane receptors and channels .

Role in Cellular Processes

PPP1R2 is particularly important during mitosis, where its activity peaks. During this phase, it regulates centrosome separation, chromosome segregation, and cytokinesis . By controlling these processes, PPP1R2 ensures proper cell division and the maintenance of genomic stability.

Research and Applications

The study of PPP1R2 has significant implications in understanding various diseases and developing therapeutic interventions. For instance, PPP1R2 is involved in pathways related to Beta-Adrenergic Signaling and the activation of cAMP-Dependent Protein Kinase A (PKA) . Additionally, mutations or dysregulation of PPP1R2 have been associated with conditions such as Sandestig-Stefanova Syndrome and Hyperlipoproteinemia, Type Id .

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THE BIOTEK
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