Introduction
PPP1R14A, also known as protein phosphatase 1 regulatory subunit 14A, functions as a phosphorylation-dependent inhibitor of smooth muscle myosin phosphatase. This inhibition leads to increased myosin phosphorylation, thereby enhancing smooth muscle contraction without increasing intracellular calcium (Ca2+) concentration. Myosin phosphatase typically reverses myosin light chain (MYL) phosphorylation to induce muscle relaxation. However, during agonist-induced contraction at constant Ca2+ levels, concurrent inhibition of myosin phosphatase by PPP1R14A results in elevated MYL phosphorylation and tension, a phenomenon known as calcium sensitization. Human pregnancy is associated with increased PKN1 expression and CPI-17 phosphorylation in the myometrium, processes in which PPP1R14A may be involved. The gene encoding PPP1R14A is located on chromosome 19q13.13-q13.2. PPP1R14A directly interacts with protein kinase C (PKC) and casein kinase I. Studies have shown that silencing PPP1R14A using siRNA reduces merlin phosphorylation, consequently affecting Ras and ERK activity in human tumor cell lines. The PKC/CPI-17 pathway, potentially modulated by PPP1R14A, plays a role in histamine-induced cytoskeletal rearrangements that compromise the lung microvascular barrier.
Description
This product consists of recombinant PPP1R14A protein produced in E. coli. It is a single, non-glycosylated polypeptide chain with 167 amino acids, resulting in a molecular mass of 18 kDa. The protein includes a 20 amino acid His Tag sequence for purification purposes and is purified using standard chromatography techniques.
Physical Appearance
Clear and colorless solution that has been sterilized by filtration.
Formulation
The PPP1R14A protein is supplied in a solution at a concentration of 1 mg/ml. The solution also contains 20 mM Tris-HCl buffer at pH 8, 0.2 mM EDTA, 1 mM DTT (dithiothreitol), and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. Adding a carrier protein, such as 0.1% HSA (human serum albumin) or BSA (bovine serum albumin), is recommended for long-term storage to maintain protein stability. Repeated freezing and thawing of the product should be avoided to prevent protein degradation.
Purity
The purity of the PPP1R14A protein is greater than 95%, as determined by SDS-PAGE analysis.
Synonyms
Protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, CPI17, CPI-17, PPP1INL, PPP1R14A.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MAAQRLGKRV LSKLQSPSRA RGPGGSPGGL QKRHARVTVK YDRRELQRRL DVEKWIDGRL EELYRGMEAD MPDEINIDEL LELESEEERS RKIQGLLKSC GKPVEDFIQE LLAKLQGLHR QPGLRQPSPS HDGSLSPLQD RARTAHP.
