PPP1R11 Human

Protein Phosphatase 1, Regulatory Subunit 11 Human Recombinant
Cat. No.
BT8567
Source
Escherichia Coli.
Synonyms
HCG-V, HCGV, IPP3, TCTE5, TCTEX5, Protein phosphatase 1 regulatory subunit 11, Hemochromatosis candidate gene V protein, PPP1R11.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PPP1R11 Human Recombinant produced in E. coli is a single polypeptide chain containing 149 amino acids (1-126) and having a molecular mass of 16.3 kDa. PPP1R11 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Protein phosphatase 1 regulatory subunit 11 (PPP1R11) is a protein encoded by the PPP1R11 gene. This gene is found within the major histocompatibility complex (MHC) class I region on chromosome 6 and is expressed in various tissues during both adulthood and embryonic development. PPP1R11 functions as an inhibitor of protein phosphatase 1 (PP1), specifically demonstrating sensitivity towards both metal-independent and metal-dependent forms of PP1. Deletion of a segment of the q arm of chromosome 6, where PPP1R11 is located, has been linked to early-onset intestinal cancer, indicating a potential role for this gene in cancer susceptibility.
Description
Recombinant human PPP1R11, expressed in E. coli, is a single polypeptide chain comprising 149 amino acids, with a molecular weight of 16.3 kDa. This protein consists of amino acids 1-126 of the PPP1R11 sequence and is fused to a 23 amino acid His-tag at the N-terminus. Purification is achieved using proprietary chromatographic techniques.
Physical Appearance
Clear solution, sterile filtered.
Formulation
The PPP1R11 solution (concentration: 0.25 mg/ml) is supplied in a buffer containing 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 20% glycerol, and 1 mM DTT.
Stability
For short-term storage (up to 2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90% based on SDS-PAGE analysis.
Synonyms
HCG-V, HCGV, IPP3, TCTE5, TCTEX5, Protein phosphatase 1 regulatory subunit 11, Hemochromatosis candidate gene V protein, PPP1R11.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAEAGAG LSETVTETTV TVTTEPENRS LTIKLRKRKP EKKVEWTSDT VDNEHMGRRS SKCCCIYEKP RAFGESSTES DEEEEEGCGH THCVRGHRKG RRRATLGPTP TTPPQPPDPS QPPPGPMQH.

Product Science Overview

Function and Mechanism

PPP1R11 is a specific inhibitor of Protein Phosphatase 1 (PP1), a major serine/threonine phosphatase that regulates a variety of cellular functions. PP1 is involved in numerous cellular processes, including cell division, protein synthesis, and muscle contraction. The regulatory subunits of PP1, such as PPP1R11, play a crucial role in targeting PP1 to specific subcellular locations and modulating its activity .

PPP1R11 exhibits differential sensitivity towards the metal-independent and metal-dependent forms of PP1. It acts as an atypical E3 ubiquitin-protein ligase, which ubiquitinates Toll-like receptor 2 (TLR2) at lysine 754, leading to its degradation by the proteasome. This process is essential for regulating inflammatory cytokine release and gram-positive bacterial clearance .

Biological Pathways

PPP1R11 is involved in several biological pathways, including Beta-Adrenergic Signaling and the Activation of cAMP-Dependent Protein Kinase A (PKA). The gene ontology annotations related to PPP1R11 include protein phosphatase inhibitor activity .

Clinical Significance

The regulation of PP1 by PPP1R11 is critical for maintaining cellular homeostasis. Dysregulation of PP1 activity can lead to various diseases, including cancer, diabetes, and neurodegenerative disorders. Understanding the role of PPP1R11 in these pathways can provide insights into potential therapeutic targets for these conditions .

Research and Applications

Recombinant human PPP1R11 is used in research to study its inhibitory effects on PP1 and its role in various cellular processes. The recombinant protein is produced using recombinant DNA technology, which involves inserting the PPP1R11 gene into an expression vector, transforming it into a host cell, and purifying the expressed protein.

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