PPP1CC Human, Active
Protein phosphatase 1 catalytic subunit gamma isozyme/isoform, Protein phosphatase 1C catalytic subunit, serine/threonine phosphatase 1 gamma, serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP1gamma, PPP1G, EC 3.1.3.16.
Greater than 85.0% as determined by SDS-PAGE.
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Description
PPP1CC produced in E.Coli is a single, non-glycosylated polypeptide chain containing 343 amino acids (1-323a.a.) and having a molecular mass of 39.1kDa.
PPP1CC is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Protein phosphatase 1 catalytic subunit gamma (PPP1CC), also known as PPP1G, is a crucial enzyme for cell division. It plays a key role in regulating protein synthesis, muscle contractions, and glycogen metabolism. PPP1CC is involved in long-term synaptic plasticity by regulating ionic conductance. It also significantly contributes to the dephosphorylation of various substrates, including the postsynaptic density-associated Ca2+/calmodulin-dependent protein kinase II.
Produced in E. coli, our PPP1CC protein is a single, non-glycosylated polypeptide chain. It consists of 343 amino acids (1-323a.a.) with a molecular weight of 39.1 kDa. This protein includes a 20 amino acid His-tag fused at the N-terminus and is purified using proprietary chromatographic techniques.
The PPP1CC protein solution is provided at a concentration of 0.25 mg/ml. It is formulated in a buffer consisting of 20 mM Tris-HCl (pH 8.0), 0.2 M NaCl, 2 mM DTT, and 50% glycerol.
The purity of PPP1CC is determined to be greater than 85.0% based on SDS-PAGE analysis.
PPP1CC exhibits a specific activity greater than 700 units/mg. This activity is measured as the enzyme's ability to hydrolyze 1.0 nanomole of p-nitrophenyl phosphate (pNPP) per minute at a pH of 7.5 and a temperature of 37°C.
Protein phosphatase 1 catalytic subunit gamma isozyme/isoform, Protein phosphatase 1C catalytic subunit, serine/threonine phosphatase 1 gamma, serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP1gamma, PPP1G, EC 3.1.3.16.
MGSSHHHHHH SSGLVPRGSH MADLDKLNID SIIQRLLEVR GSKPGKNVQL QENEIRGLCL KSREIFLSQP ILLELEAPLK ICGDIHGQYY DLLRLFEYGG FPPESNYLFL GDYVDRGKQS LETICLLLAY KIKYPENFFL LRGNHECASI NRIYGFYDEC KRRYNIKLWK TFTDCFNCLP IAAIVDEKIF CCHGGLSPDL QSMEQIRRIM RPTDVPDQGL LCDLLWSDPD KDVLGWGEND RGVSFTFGAE VVAKFLHKHD LDLICRAHQV VEDGYEFFAK RQLVTLFSAP NYCGEFDNAG AMMSVDETLM CSFQILKPAE KKKPNATRPV TPPRGMITKQ AKK.
Product Science Overview
Protein Phosphatase 1 (PP1) is a major serine/threonine phosphatase that plays a crucial role in various cellular processes. The catalytic subunit gamma (PP1C gamma) is one of the isoforms of PP1, encoded by the PPP1CC gene in humans . This enzyme is involved in the regulation of numerous cellular functions, including glycogen metabolism, muscle contraction, cell division, and neuronal activities .
The catalytic subunit of PP1 is a highly conserved protein among eukaryotes, suggesting a common catalytic mechanism . The structure of PP1C gamma consists of a 30-kilodalton single-domain protein that forms complexes with various regulatory subunits . These regulatory subunits are essential for substrate specificity and compartmentalization within the cell .
The catalytic mechanism of PP1 involves the coordination of metal ions, such as manganese and iron, which are essential for its activity . The enzyme uses a di-metal ion mechanism to activate water molecules, which then initiate a nucleophilic attack on the phosphorus atom of the substrate .
PP1C gamma is involved in a wide range of cellular processes, including:
- Glycogen Metabolism: PP1 plays a key role in the interconversion between phosphorylase a and b, which is crucial for glycogen metabolism .
- Muscle Contraction: PP1 regulates muscle contraction by dephosphorylating myosin light chains .
- Cell Division and Mitosis: PP1 is essential for the progression of the cell cycle and mitosis .
- Neuronal Activities: PP1 is involved in the regulation of neuronal functions, including synaptic plasticity and memory formation .
The activity of PP1C gamma is regulated by its interaction with various regulatory subunits. These subunits target PP1C gamma to specific subcellular locations and modulate its activity and substrate specificity . The regulatory subunits often contain a conserved binding motif known as the RVxF motif, which interacts with a hydrophobic groove on the surface of PP1C gamma .
