PPP1CA Human
Description
PPP1CA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
The PPP1CA gene is located on chromosome 11 in humans . It encodes the alpha isoform of the catalytic subunit of PP1, which is one of the three catalytic subunits of the enzyme. The protein is broadly expressed and associates with over 200 regulatory proteins to form holoenzymes, which dephosphorylate their biological targets with high specificity .
PP1 is involved in the regulation of several critical cellular processes, including:
- Cell Division: PP1 plays a role in the progression of the cell cycle and mitosis.
- Glycogen Metabolism: It regulates glycogen synthesis and breakdown.
- Muscle Contractility: PP1 is involved in the regulation of muscle contraction.
- Protein Synthesis: It influences the translation of proteins.
- HIV-1 Viral Transcription: PP1 is implicated in the transcription of HIV-1 .
Increased activity of PP1 has been observed in the end stage of heart failure, suggesting its importance in cardiac function. Deregulation of PP1 is also implicated in diabetes and multiple types of cancer .
The recombinant form of Protein Phosphatase 1, Catalytic Subunit Alpha, is produced using recombinant DNA technology. This involves cloning the PPP1CA gene into an expression vector, which is then introduced into a host cell (such as E. coli) to produce the protein. The recombinant protein is then purified for use in research and therapeutic applications .
