PPM1A Antibody

Protein Phosphatase 1A Alpha Isoform, Mouse Anti Human
Cat. No.
BT23687
Source
Synonyms
Protein phosphatase 1A, EC 3.1.3.16, Protein phosphatase 2C isoform alpha, PP2C-alpha, IA, PPM1A, PP2CA, MGC9201.
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Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
Protein Phosphatase 2C alpha (PP2Cα), a member of the PP2C family of Ser/Thr protein phosphatases, acts as a negative regulator of cellular stress response pathways. This phosphatase dephosphorylates and negatively regulates the activities of MAP kinases and MAP kinase kinases, effectively inhibiting the activation of p38 and JNK kinase cascades triggered by environmental stresses. PP2Cα also dephosphorylates cyclin-dependent kinases, suggesting a role in cell cycle control. Overexpression of this phosphatase has been shown to activate the expression of the tumor suppressor gene TP53/p53, leading to G2/M cell cycle arrest and apoptosis. Three alternatively spliced transcript variants encoding two distinct isoforms of PP2Cα have been identified.
Formulation
The antibody is supplied at a concentration of 1mg/ml in a solution of PBS at pH 7.4 with 0.1% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), the antibody can be stored at 4°C. For long-term storage, it is recommended to store at -20°C. Repeated freeze-thaw cycles should be avoided.
Stability / Shelf Life
The antibody has a shelf life of 12 months when stored at -20°C and 1 month when stored at 4°C.
Applications
The PPM1A antibody has been validated by ELISA and Western blot analysis to ensure its specificity and reactivity. However, optimal working dilutions should be determined empirically for each application. For Western blot analysis, a dilution range of 1:250 to 1:500 is recommended, with a starting dilution of 1:250.
Synonyms
Protein phosphatase 1A, EC 3.1.3.16, Protein phosphatase 2C isoform alpha, PP2C-alpha, IA, PPM1A, PP2CA, MGC9201.
Purification Method
PPM1A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
Pp6c7AT.
Immunogen
Anti-human PPM1A mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPM1A amino acids 1-382 purified from E. coli.
Ig Subclass
Mouse IgG2b heavy chain and κ light chain.

Product Science Overview

Introduction

Protein Phosphatase 1A Alpha Isoform (PPM1A), also known as Protein Phosphatase 2C Alpha (PP2C-alpha), is a member of the PP2C family of Ser/Thr protein phosphatases. These enzymes are critical in regulating various cellular processes by dephosphorylating target proteins, thus modulating their activity.

Structure and Function

PPM1A is encoded by the PPM1A gene and is involved in the negative regulation of cell stress response pathways. It achieves this by dephosphorylating and inactivating MAP kinases and MAP kinase kinases, which are key players in cellular stress responses . The enzyme’s activity is crucial for maintaining cellular homeostasis and preventing excessive stress responses that could lead to cell damage or death.

Mouse Anti-Human PPM1A Antibody

The mouse anti-human PPM1A antibody is a monoclonal antibody derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PPM1A . This antibody is used in various research applications, including Western blotting (WB), immunohistochemistry (IHC-P), and immunocytochemistry/immunofluorescence (ICC/IF) .

Applications in Research

The mouse anti-human PPM1A antibody is widely used in scientific research to study the role of PPM1A in cellular processes. It helps in:

  • Identifying and quantifying PPM1A expression in different tissues and cell types.
  • Investigating the regulatory mechanisms of PPM1A in stress response pathways.
  • Exploring the potential therapeutic targets for diseases related to dysregulated stress responses, such as cancer and neurodegenerative disorders.

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Protein Phosphatase 1A Alpha Isoform Human Recombinant
Cat. No.
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Source
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THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
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