PODXL Mouse
Description
Product Specs
Product Science Overview
Podocalyxin is characterized by its complex structure, which includes:
- Extracellular Domain (ECD): Comprising 406 amino acids, this domain is heavily glycosylated and is crucial for the protein’s interaction with other cellular components.
- Transmembrane Segment: A 26 amino acid segment that anchors the protein to the cell membrane.
- Cytoplasmic Domain: A 75 amino acid segment that is involved in intracellular signaling .
The primary function of Podocalyxin is to maintain the structure and function of the glomerular filtration barrier in the kidneys. It is expressed on the apical surface of podocytes, which are specialized cells in the kidney glomerulus. Podocalyxin’s negative charge helps to repel negatively charged molecules, preventing their passage through the filtration barrier.
Podocalyxin has been identified as a significant marker in various cancers. Its expression is upregulated in tumors with a high metastatic index, and its presence is often associated with poor prognosis . In human tumor xenograft models, Podocalyxin expression promotes tumor growth and metastasis. This makes it an important prognostic and theragnostic marker in several human cancers .
Recombinant Podocalyxin, such as the mouse recombinant version, is produced using advanced biotechnological methods. This involves cloning the gene encoding Podocalyxin and expressing it in a suitable host system, such as a mouse myeloma cell line. The recombinant protein is then purified and characterized for use in various research applications .
Recombinant Podocalyxin is widely used in research to study its role in cell adhesion, migration, and cancer progression. It is also used to develop targeted therapies for cancer treatment. For instance, novel antibodies targeting Podocalyxin have been developed to selectively recognize tumor-restricted glycoepitopes on the extracellular mucin domain of Podocalyxin . These antibodies have shown promising results in preclinical studies, demonstrating specific efficacy in killing tumor cells.
