PODXL Human
HEK293 Cells
Podocalyxin, Podocalyxin-like protein 1, PC, PCLP-1.
Description
PODXL Human Recombinant produced in HEK293 cells is a single, glycosylated polypeptide chain containing 416 amino acids (23-429 a.a.) and having a molecular mass of 43.1 kDa. PODXL is expressed with a 6 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Podocalyxin, Podocalyxin-like protein 1, PC, PCLP-1.
HEK293 Cells
DGS-SPSPSPS PSQNATQTTT DSSNKTAPTP ASSVTIMATD TAQQSTVPTS KANEILASVK ATTLGVSSDS PGTTTLAQQV SGPVNTTVAR GGGSGNPTTT IESPKSTKSA DTTTVATSTA TAKPNTTSSQ NGAEDTTNSG GKSSHSVTTD LTSTKAEHLT TPHPTSPLSP RQPTSTHPVA TPTSSGHDHL MKISSSSSTV AIPGYTFTSP GMTTTLPSSV ISQRTQQTSS QMPASSTAPS SQETVQPTSP ATALRTPTLP ETMSSSPTAA STTHRYPKTP SPTVAHESNW AKCEDLETQT QSEKQLVLNL TGNTLCAGGA SDEKLISLIC RAVKATFNPA QDKCGIRLAS VPGSQTVVVK EITIHTKLPA KDVYERLKDK WDELKEAGVS DMKLGDQGPP EEAEDRFSMP-HHHHHH.
Product Science Overview
Mature human Podocalyxin consists of:
- A 406 amino acid extracellular domain (ECD)
- A 26 amino acid transmembrane segment
- A 75 amino acid cytoplasmic domain
The recombinant form of Podocalyxin is typically produced in a mouse myeloma cell line (NS0-derived) and includes a C-terminal Asp and 10-His tag .
Podocalyxin is primarily expressed in the kidney, where it plays a vital role in maintaining the structure and function of the glomerular filtration barrier. It is also found in vascular endothelial cells, hematopoietic stem cells, and certain types of cancer cells .
In the context of cancer, Podocalyxin has been associated with an aggressive tumor phenotype and poor prognosis in several cancer types, including colorectal cancer . Its overexpression is linked to increased tumor cell migration and invasion, making it a potential biomarker for cancer prognosis and a target for therapeutic intervention .
Recombinant human Podocalyxin is used in various research applications, including:
- Cell adhesion studies: It has been shown to inhibit the adhesion of CHO (Chinese hamster ovary) cells transfected with P-Selectin .
- Cancer research: Due to its role in tumor progression, Podocalyxin is studied as a potential target for cancer therapy .
- Stem cell research: As a marker for hematopoietic stem cells, it is used in studies related to stem cell biology and regenerative medicine .
Recombinant human Podocalyxin is typically lyophilized from a 0.2 μm filtered solution in PBS and should be reconstituted at 100 μg/mL in PBS. It is stable for 12 months from the date of receipt when stored at -20 to -70 °C, and for 1 month at 2 to 8 °C under sterile conditions after reconstitution .
