PI3Kb Human
Description
Product Specs
Product Science Overview
Phosphoinositide 3-kinases (PI3Ks) are a family of enzymes involved in various cellular functions, including cell growth, proliferation, differentiation, motility, survival, and intracellular trafficking . Among the different classes of PI3Ks, Class I PI3Ks are particularly significant due to their role in activating protein kinase B (PKB/AKT), which is crucial for cell survival, proliferation, and differentiation .
Class I PI3Ks are heterodimeric molecules composed of a regulatory subunit (p85) and a catalytic subunit (p110). The p110β subunit, in particular, interacts with the p85α regulatory subunit to form the PI3K beta complex . This complex is involved in the phosphorylation of the 3’ position hydroxyl group of the inositol ring of phosphatidylinositol (PtdIns), leading to the production of phosphatidylinositol (3,4,5)-trisphosphate (PIP3) .
The PI3K/AKT signaling pathway is a vital regulatory component in human tissues, influencing metabolism, cell survival, and neuronal functions . The activation of PI3K leads to the production of PIP3, which in turn activates AKT. Activated AKT then phosphorylates various downstream targets involved in cell growth, survival, and metabolism .
The PI3K/AKT pathway is often dysregulated in cancer, leading to elevated PI3K signaling and altered cellular processes that contribute to tumor development . The p110β subunit, along with p110α, has been identified as a potential target for overcoming multidrug resistance (MDR) in cancer . Inhibiting the activation of these subunits can downregulate ATP-binding cassette transporters like P-gp/ABCB1 and BCRP/ABCG2, thereby reestablishing drug sensitivity in cancer cells .
Human recombinant PI3K beta p110β/p85α is a laboratory-produced version of the naturally occurring enzyme complex. It is used in research to study the PI3K/AKT signaling pathway and its implications in various diseases, including cancer. By using recombinant proteins, researchers can better understand the structure, function, and regulatory mechanisms of PI3Ks, leading to the development of targeted therapies for diseases associated with PI3K dysregulation .
