PI3Ka Bovine

Phosphoinositide 3-kinase alpha p110a/p85a Bovine Recombinant
Cat. No.
BT1765
Source
Sf9 insect cells.
Synonyms
Phosphoinositide 3-kinase alpha p110a/p85a, PI3Ka, 2.7.1.137, Phosphatidylinositol 3-kinase, 1-phosphatidylinositol 3-kinase, PI3-kinase, PtdIns-3-kinase, Type I phosphatidylinositol kinase, Type III phosphoinositide 3-kinase.
Appearance
Sterile filtered liquid formualtion.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
Prospec's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

The PI3Ka catalytic and regulatory subunits are coexpressed in Sf9 insect cells. Phosphoinositide 3-kinase alpha Bovine Recombinant is a glycosilated protein having a molecular weight as follows: p85a chain 83.5 kDa, p110 chain 124.3 kDa.

Product Specs

Introduction
PI3Ka plays a crucial part in the programmed death of human colon cancer cells. When adenocarcinoma cells were treated with antibodies that neutralize PI3Ka, apoptosis was triggered. However, this effect was reversed when the cells were exposed to a caspase inhibitor. Research has also revealed that PI3Ka-mediated phosphorylation of the p85a adapter molecule diminishes the lipid kinase activity within the heterodimer. This finding suggests that PI3K-dependent signaling processes might not always rely on the production of 3'-phosphorylated phosphoinositides. Moreover, PI3Ka is essential for initiating keratinocyte differentiation. Any reduction in PI3Ka activity leads to a decline in keratinocyte adherence to the extracellular matrix, thereby triggering the early stages of differentiation.
Description
The catalytic and regulatory subunits of PI3Ka are simultaneously produced in Sf9 insect cells. This recombinant bovine PI3Ka is a glycosylated protein. Its p85a chain has a molecular weight of 83.5 kDa, while the p110 chain weighs 124.3 kDa.
Physical Appearance
The product is a sterile, filtered liquid.
Formulation
PI3Ka is supplied in a solution containing 25mM HEPES (pH 8.0), 25mM NaCl, 2.5mM MgCl2, and 50% glycerol.
Stability
For optimal storage, keep the product at 4°C if the entire vial will be used within 2-4 weeks. For prolonged storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advised for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
SDS-PAGE analysis indicates a purity exceeding 90%.
Unit Definition
One unit corresponds to the transfer of one picomole of phosphate to PIP2 in one minute.
Specific Activity
The specific activity is 1,000 units per milligram.
Synonyms
Phosphoinositide 3-kinase alpha p110a/p85a, PI3Ka, 2.7.1.137, Phosphatidylinositol 3-kinase, 1-phosphatidylinositol 3-kinase, PI3-kinase, PtdIns-3-kinase, Type I phosphatidylinositol kinase, Type III phosphoinositide 3-kinase.
Source
Sf9 insect cells.

Product Science Overview

Introduction

Phosphoinositide 3-kinases (PI3Ks) are a family of enzymes involved in various cellular functions, including cell growth, proliferation, differentiation, motility, survival, and intracellular trafficking. These enzymes play a crucial role in cellular signaling pathways and are implicated in cancer and other diseases .

Structure and Function

Phosphoinositide 3-kinase alpha (PI3Kα) is a class I PI3K composed of a catalytic subunit (p110α) and a regulatory subunit (p85α). The p110α subunit is encoded by the PIK3CA gene, while the p85α subunit is encoded by the PIK3R1 gene . The catalytic subunit p110α uses ATP to phosphorylate phosphatidylinositols (PtdIns), PtdIns4P, and PtdIns(4,5)P2 .

Role in Cancer

The involvement of p110α in human cancer has been hypothesized since 1995. Genetic and functional studies have shown that common activating PIK3CA missense mutations are present in various human tumors . These mutations are particularly prevalent in breast cancers, with enrichment in the luminal and human epidermal growth factor receptor 2-positive subtypes (HER2+) . The three hotspot mutation positions (GLU542, GLU545, and HIS1047) have been widely reported .

Bovine Recombinant PI3Kα

Phosphoinositide 3-kinase alpha Bovine Recombinant is a glycosylated protein with a molecular weight of approximately 83.5 kDa for the p85α chain and 124.3 kDa for the p110α chain . This recombinant protein is used in various research applications to study the PI3K pathway and its role in cellular signaling and cancer.

Clinical Implications

Due to the association between p110α and cancer, it is considered an appropriate drug target. Pharmaceutical companies are designing and characterizing potential p110α isoform-specific inhibitors . The presence of PIK3CA mutations may also predict the response to certain therapies, such as aspirin therapy for colorectal cancer .

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