PHOSPHO2 is a single, non-glycosylated polypeptide chain containing 265 amino acids (1-241) and has a molecular mass of 30.3 kDa . The recombinant form of PHOSPHO2 is produced in Escherichia coli (E. coli) and is fused to a 24 amino acid His-tag at the N-terminus . This fusion allows for easier purification using chromatographic techniques.
PHOSPHO2 shares a 42% sequence identity with another phosphatase, PHOSPHO1 . Both enzymes are involved in the hydrolysis of phosphoethanolamine and phosphocholine, although recombinant PHOSPHO2 hydrolyzes these substrates relatively poorly compared to PHOSPHO1 . PHOSPHO1 is upregulated in mineralizing cells and is believed to play a role in the production of inorganic phosphate for bone mineralization .
The PHOSPHO2 solution is typically formulated in a buffer containing 20 mM Tris-HCl (pH 8.0), 1 mM DTT, 10% glycerol, and 0.1 M NaCl . For short-term storage, it is recommended to keep the solution at 4°C, while for long-term storage, it should be frozen at -20°C . To prevent degradation, it is advisable to avoid multiple freeze-thaw cycles and to add a carrier protein such as 0.1% HSA or BSA for extended storage .
PHOSPHO2 is primarily used in laboratory research and is not intended for use as a drug, agricultural product, food additive, or household chemical . Its role in research is crucial for understanding the biochemical pathways involving phosphoethanolamine and phosphocholine, as well as its potential implications in bone mineralization processes.