PHB Human
Description
PHB is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Prohibitin (PHB) is a highly conserved protein that plays a crucial role in various cellular processes, including cell cycle regulation, apoptosis, and mitochondrial function. It is part of the prohibitin family, which includes two main members: prohibitin-1 (PHB1) and prohibitin-2 (PHB2). These proteins are known for their ability to form large complexes within the mitochondria, where they help maintain mitochondrial integrity and function. Recombinant human prohibitin is a form of this protein that is produced through recombinant DNA technology, allowing for its use in various research and therapeutic applications.
Recombinant human prohibitin is typically produced using bacterial or mammalian expression systems. The gene encoding prohibitin is cloned into an expression vector, which is then introduced into the host cells. These cells are cultured under conditions that promote the expression of the prohibitin protein. Once expressed, the protein is purified using techniques such as affinity chromatography, which ensures a high degree of purity and activity.
Prohibitin is involved in several key biochemical pathways and interactions. It acts as a scaffold protein, facilitating the assembly of multi-protein complexes that are essential for mitochondrial function. Prohibitin interacts with various signaling molecules, including kinases and phosphatases, to regulate cellular processes such as apoptosis and cell proliferation. Additionally, prohibitin has been shown to play a role in the regulation of mitochondrial respiration and the maintenance of mitochondrial DNA.
The expression and activity of prohibitin are tightly regulated at multiple levels. Transcriptional regulation involves various transcription factors that bind to the promoter region of the prohibitin gene, modulating its expression in response to cellular signals. Post-translational modifications, such as phosphorylation and ubiquitination, also play a critical role in regulating prohibitin’s function and stability. Furthermore, prohibitin can be regulated through its interactions with other proteins, which can influence its localization and activity within the cell.
