PFN4 Human
Profilin-4 Human Recombinant
Cat. No.
BT7396
Source
Escherichia Coli.
Synonyms
PFN-4, Profilin-IV, Profilin4.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PFN4 Human Recombinant produced in E.Coli is a single, non-glycosylated,
polypeptide chain containing 149 amino acids (1-129 a.a.) and having a molecular mass of 16.4 kDa. PFN4 protein is fused to a 20 amino acid His tag at N-terminus and purified by standard chromatography.
Product Specs
Introduction
Profilin 4 (PFN4) is a small actin-binding protein that plays a vital role in the dynamic regulation of the actin cytoskeleton. Found in most cells across eukaryotic organisms, PFN4 is essential for the controlled growth of actin microfilaments, a process crucial for cell movement and shape changes.
Description
Recombinant human PFN4, expressed in E. coli, is a single, non-glycosylated polypeptide chain comprising 149 amino acids (residues 1-129) with a molecular weight of 16.4 kDa. The protein includes a 20 amino acid His tag at the N-terminus and is purified using standard chromatography techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The human PFN4 solution is prepared in a buffer containing 20mM Tris-HCl at pH 8, 1mM DTT, and 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For longer storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for extended storage. Repeated freezing and thawing should be avoided.
Purity
Purity is determined to be greater than 95% based on SDS-PAGE analysis.
Synonyms
PFN-4, Profilin-IV, Profilin4.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSHLQSLLLD TLLGTKHVDS AALIKIQERS LCVASPGFNV TPSDVRTLVN GFAKNPLQAR REGLYFKGKD YRCVRADEYS LYAKNENTGV VVVKTHLYLL VATYTEGMYP SICVEATESL GDYLRKKGS.
Product Science Overview
Structure and Expression
Function
Unlike other profilin family members, Profilin-4 does not bind to actin . Instead, it binds to various phosphatidylinositol phosphates, including phosphatidylinositol 3-phosphate (PtdIns(3)P), phosphatidylinositol 4,5-bisphosphate (PtdIns(4,5)P2), and phosphatidic acid (PA) . This binding is crucial for its role in the spatial and temporal regulation of actin microfilament growth .
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