PFN2 Human

Profilin-2 is a 140 amino acid protein with a molecular weight of approximately 17.2 kDa . It is expressed in Escherichia coli (E. coli) for recombinant production and is typically purified to a high degree of purity (>95%) using conventional chromatography techniques . The recombinant protein often includes an N-terminal His-tag to facilitate purification .

Profilin-2 binds to actin monomers (G-actin) and regulates actin polymerization, which is essential for various cellular processes such as cell motility, division, and signaling . At high concentrations, profilin-2 prevents the polymerization of actin, whereas at low concentrations, it enhances actin polymerization . This dual role allows profilin-2 to finely tune the dynamics of the actin cytoskeleton in response to extracellular signals.

Additionally, profilin-2 interacts with phosphatidylinositol 4,5-bisphosphate (PIP2), inhibiting the formation of inositol trisphosphate (IP3) and diacylglycerol (DG), which are important secondary messengers in cellular signaling pathways .

Recombinant human profilin-2 is widely used in research to study the regulation of the actin cytoskeleton and its role in various cellular processes. It is also used in biochemical assays to investigate protein-protein interactions and the effects of profilin-2 on actin dynamics .

Recombinant human profilin-2 is typically stored at 4°C for short-term use and at -20°C for long-term storage. It is important to avoid freeze-thaw cycles to maintain the protein’s stability and activity .