PECAM1 Human

Platelet Endothelial Cell Adhesion Molecule 1 Human Recombinant
Cat. No.
BT30588
Source
Sf9, Baculovirus cells.
Synonyms
Platelet endothelial cell adhesion molecule, PECAM1, CD31, CD31/EndoCAM, endoCAM, GPIIA', PECA1, PECAM-1, Platelet Endothelial Cell Adhesion Molecule 1.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. They may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PECAM1 Human Recombinant produced in Sf9 Baculovirus is a single, non-glycosylated polypeptide chain containing 582 amino acids (28-601a.a.) and having a molecular mass of 65.5kDa (Migrates at 70-100kDa on SDS-PAGE under reducing conditions).
PECAM1 is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. 

Product Specs

Introduction
Platelet endothelial cell adhesion molecule (PECAM1) plays a critical role in atherosclerosis development. It prevents phagocytes from engulfing nearby healthy cells by sending detachment signals. Upon apoptosis (cell death), PECAM1's function changes, promoting the binding of dying cells to phagocytes. When a healthy cell interacts with a phagocyte through the homophilic binding of PECAM1 on both cell surfaces, the healthy cell actively repels the phagocyte.
Description
Recombinant human PECAM1, produced in Sf9 Baculovirus, is a single, non-glycosylated polypeptide chain consisting of 582 amino acids (28-601a.a.). It has a molecular mass of 65.5 kDa and appears as a band between 70-100 kDa on SDS-PAGE under reducing conditions. The PECAM1 protein has an 8 amino acid His-tag fused at its C-terminus and is purified using proprietary chromatographic methods.
Physical Appearance
Clear, colorless solution that has been sterilized by filtration.
Formulation
The PECAM1 protein solution has a concentration of 0.25 mg/ml and is prepared in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), the protein solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. To ensure optimal stability during long-term storage, consider adding a carrier protein (0.1% HSA or BSA). Repeated freezing and thawing of the solution should be avoided.
Purity
The purity of the PECAM1 protein is greater than 95.0% as assessed by SDS-PAGE analysis.
Synonyms
Platelet endothelial cell adhesion molecule, PECAM1, CD31, CD31/EndoCAM, endoCAM, GPIIA', PECA1, PECAM-1, Platelet Endothelial Cell Adhesion Molecule 1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
QENSFTINSV DMKSLPDWTV QNGKNLTLQC FADVSTTSHV KPQHQMLFYK DDVLFYNISS MKSTESYFIP EVRIYDSGTY KCTVIVNNKE KTTAEYQVLV EGVPSPRVTL DKKEAIQGGI VRVNCSVPEE KAPIHFTIEK LELNEKMVKL KREKNSRDQN FVILEFPVEE QDRVLSFRCQ ARIISGIHMQ TSESTKSELV TVTESFSTPK FHISPTGMIM EGAQLHIKCT IQVTHLAQEF PEIIIQKDKA IVAHNRHGNK AVYSVMAMVE HSGNYTCKVE SSRISKVSSI VVNITELFSK PELESSFTHL DQGERLNLSC SIPGAPPANF TIQKEDTIVS QTQDFTKIAS KSDSGTYICT AGIDKVVKKS NTVQIVVCEM LSQPRISYDA QFEVIKGQTI EVRCESISGT LPISYQLLKT SKVLENSTKN SNDPAVFKDN PTEDVEYQCV ADNCHSHAKM LSEVLRVKVI APVDEVQISI LSSKVVESGE DIVLQCAVNE GSGPITYKFY REKEGKPFYQ MTSNATQAFW TKQKASKEQE GEYYCTAFNR ANHASSVPRS KILTVRVILA PWKKVEHHHH HH.

Product Science Overview

Structure and Location

PECAM-1 is a highly glycosylated protein with a molecular mass of approximately 130 kDa. It consists of an N-terminal domain with 574 amino acids, a transmembrane domain with 19 amino acids, and a C-terminal cytoplasmic domain with 118 amino acids . The PECAM1 gene is located on chromosome 17q23.3 in humans .

Expression and Function

PECAM-1 is expressed on the surface of platelets, monocytes, neutrophils, and certain types of T-cells. It constitutes a significant portion of endothelial cell intercellular junctions . This protein is involved in several key functions:

  1. Leukocyte Transendothelial Migration (TEM): PECAM-1 is essential for the migration of leukocytes across the endothelial barrier during inflammatory responses .
  2. Angiogenesis: PECAM-1 plays a role in the formation of new blood vessels from pre-existing ones .
  3. Integrin Activation: It is involved in the activation of integrins, which are important for cell adhesion and signaling .
Mechanism of Action

PECAM-1 mediates its functions through both homophilic and heterophilic interactions. Homophilic interactions involve PECAM-1 binding to itself on adjacent cells, which is important for endothelial cell-cell adhesion . Heterophilic interactions, such as with CD177, facilitate the transendothelial migration of neutrophils .

Clinical Significance

PECAM-1 is implicated in various diseases and conditions. For instance, it is associated with angiosarcoma, a type of cancer that originates in the lining of blood vessels . Additionally, PECAM-1 plays a role in the immune response and has been linked to conditions involving inflammation and immune cell migration .

Recombinant PECAM-1

Recombinant PECAM-1 is produced using recombinant DNA technology, which involves inserting the PECAM1 gene into a suitable expression system to produce the protein in vitro. This recombinant form is used in research to study the protein’s structure, function, and role in various physiological and pathological processes.

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Cat. No.
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Source
Sf9, Baculovirus cells.
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