PECAM1 Mouse
Greater than 90% as determined by SDS-PAGE.
Description
PECAM1 Mouse Recombinant produced in HEK293 is a single, non-glycosylated polypeptide chain containing 579 amino acids (18-590 a.a.) and having a molecular mass of 65.3 kDa. PECAM1 Mouse is fused to an 6 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
EENSFTINSI HMESLPSWEV MNGQQLTLEC LVDISTTSKS RSQHRVLFYK DDAMVYNVTS REHTESYVIP QARVFHSGKY KCTVMLNNKE KTTIEYEVKV HGVSKPKVTL DKKEVTEGGV VTVNCSLQEE KPPIFFKIEK LEVGTKFVKR RIDKTSNENF VLMEFPIEAQ DHVLVFRCQA GILSGFKLQE SEPIRSEYVT VQESFSTPKF EIKPPGMIIE GDQLHIRCIV QVTHLVQEFT EIIIQKDKAI VATSKQSSEA VYSVMAMVEY SGHYTCKVES NRISKASSIM VNITELFPKP KLEFSSSRLD QGELLDLSCS VSGTPVANFT IQKEETVLSQ YQNFSKIAEE SDSGEYSCTA GIGKVVKRSG LVPIQVCEML SKPSIFHDAK SEIIKGHAIG ISCQSENGTA PITYHLMKAK SDFQTLEVTS NDPATFTDKP TRDMEYQCRA DNCHSHPAVF SEILRVRVIA PVDEVVISIL SSNEVQSGSE MVLRCSVKEG TSPITFQFYK EKEDRPFHQA VVNDTQAFWH NKQASKKQEG QYYCTASNRA SSMRTSPRSS TLAVRVFLAP WKK HHHHH
Product Science Overview
Platelet Endothelial Cell Adhesion Molecule 1 (PECAM-1), also known as CD31, is a protein encoded by the PECAM1 gene. It is a member of the immunoglobulin superfamily and is primarily found on the surface of platelets, monocytes, neutrophils, and certain types of T-cells. PECAM-1 plays a crucial role in various physiological processes, including leukocyte migration, angiogenesis, and integrin activation .
PECAM-1 is a 130-kilodalton glycoprotein that consists of six extracellular immunoglobulin-like domains, a transmembrane domain, and a cytoplasmic tail containing immunoreceptor tyrosine-based inhibitory motifs (ITIMs). These ITIMs are essential for recruiting and activating protein-tyrosine phosphatases, such as SHP-1 and SHP-2 .
In mice, PECAM-1 is expressed at the lateral junctions of endothelial cells and on the surface of various hematopoietic cells, including monocytes, neutrophils, natural killer cells, platelets, and naive T and B cells . The expression of PECAM-1 is crucial for maintaining the integrity of endothelial cell junctions and facilitating cell-cell adhesion .
PECAM-1 is involved in several key biological functions:
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Leukocyte Transendothelial Migration (TEM): PECAM-1 is required for the efficient migration of leukocytes across endothelial barriers during inflammatory responses. It facilitates the trafficking of leukocytes to and from the lateral border recycling compartment (LBRC), which is essential for TEM .
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Angiogenesis: PECAM-1 plays a role in the formation of new blood vessels by promoting endothelial cell-cell adhesion and signaling .
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Integrin Activation: PECAM-1 is involved in the activation of integrins, which are essential for cell adhesion and signaling .
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Regulation of B-cell Development: PECAM-1 acts as a regulator of B-cell development and activation. PECAM-1-deficient mice exhibit a hyperresponsive B-cell phenotype, increased numbers of B-1 cells, and reduced B-2 cells. These mice also develop autoantibodies and lupus-like autoimmune disease with age .
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Negative Regulation of Platelet-Collagen Interactions: PECAM-1 negatively regulates platelet-collagen interactions, which are crucial for platelet adhesion and aggregation during blood clotting .
Recombinant PECAM-1, particularly from mouse sources, is widely used in research to study its functions and interactions. Recombinant proteins are produced using various expression systems, such as bacterial, yeast, insect, or mammalian cells. These proteins are then purified and used in various assays to investigate the role of PECAM-1 in different biological processes.
