PDCL Human

The PDCL gene is located on chromosome 9 at the band 9q33.2 . The gene spans approximately 30,000 base pairs and consists of multiple exons. The protein encoded by this gene is involved in various cellular processes, including the regulation of G-protein-coupled receptor (GPCR) signaling pathways .

Phosducin-like protein plays a crucial role in the modulation of heterotrimeric G proteins. It is a putative modulator that shares extensive amino acid sequence homology with phosducin . The primary function of phosducin-like protein is to regulate G-protein signaling by binding to the beta-gamma subunits of G proteins . This interaction is essential for the proper functioning of GPCR signaling pathways, which are involved in various physiological processes such as vision, taste, and neurotransmission .

Phosducin-like protein is expressed in various tissues, including the retina, pineal gland, and other parts of the central nervous system . The protein is predominantly localized in the cytoplasm and cytosol, where it interacts with other proteins to modulate their functions . Additionally, phosducin-like protein has been shown to be involved in the regulation of the cytoskeleton and cell projection organization .

Recombinant phosducin-like protein is produced using recombinant DNA technology, which involves inserting the PDCL gene into an expression vector and introducing it into a host cell, such as Escherichia coli or mammalian cells. The host cells then produce the protein, which can be purified and used for various research and therapeutic applications .

Phosducin-like protein has been the subject of extensive research due to its role in G-protein signaling and its potential therapeutic applications. Studies have shown that modulation of phosducin-like protein levels can impact cytoskeletal remodeling and cell morphology . This protein is also being investigated for its potential role in the treatment of diseases related to GPCR signaling dysregulation, such as certain types of cancer and neurological disorders .

In conclusion, phosducin-like protein is a critical modulator of G-protein signaling with significant implications for various physiological processes and potential therapeutic applications. The recombinant form of this protein provides valuable insights into its functions and potential uses in research and medicine.