PARK7 P1E12AT Antibody

Parkinson Disease Protein 7 Clone P1E12AT, Mouse Anti Human
Cat. No.
BT28742
Source
Synonyms
PARK7, Parkinson disease protein 7, Oncogene DJ-1, Parkinson disease (autosomal recessive, early onset) 7.
Appearance
Sterile filtered colorless solution.
Purity
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

Product Specs

Introduction
PARK7 is a ubiquitously expressed protein with roles in various cellular processes, including spermatogenesis, fertilization, cancer, RNA-binding, androgen-receptor signaling, and oxidative stress. Mutations in PARK7 are linked to autosomal recessive early-onset Parkinson's disease 7 (Park7).
Physical Appearance
A colorless solution that has been sterilized by filtration.
Formulation
The solution contains 1mg/ml of PARK7 P1E12AT Antibody in a buffer of PBS at pH 7.4, with 10% Glycerol and 0.02% Sodium Azide.
Storage Procedures
For short-term storage (up to 1 month), keep at 4°C. For longer storage, store at -20°C. Avoid repeated freeze-thaw cycles.
Stability / Shelf Life
The antibody is stable for 12 months when stored at -20°C, and for 1 month at 4°C.
Applications
This antibody has undergone testing by ELISA and Western blot analysis, confirming its specificity and reactivity. As application requirements can differ, it is recommended to titrate the antibody for optimal results in each specific investigation. A starting dilution of 1:1000 is advised.
Synonyms
PARK7, Parkinson disease protein 7, Oncogene DJ-1, Parkinson disease (autosomal recessive, early onset) 7.
Purification Method
PARK7 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
Type
Mouse Anti Human Monoclonal.
Clone
P1E12AT.
Immunogen
Anti-human PARK7 mAb, clone P1E12AT, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human PARK7 protein 1-189 amino acids purified from E. coli
Ig Subclass
Mouse IgG2b heavy chain and k light chain.

Product Science Overview

Introduction

Parkinson Disease Protein 7 (PARK7), also known as Protein DJ-1, is a ubiquitously expressed protein involved in various cellular processes. It plays a significant role in spermatogenesis, fertilization, cancer, RNA-binding, androgen-receptor signaling, and oxidative stress response . Mutations in the PARK7 gene are known to cause autosomal recessive early-onset Parkinson’s disease (Park7) .

Clone P1E12AT

Clone P1E12AT is a specific monoclonal antibody targeting the human PARK7 protein. This antibody is derived from the hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human PARK7 protein (amino acids 1-189) purified from E. coli .

Characteristics
  • Type: Mouse Anti Human Monoclonal Antibody
  • Ig Subclass: Mouse IgG 2b heavy chain and κ light chain
  • Purification Method: The antibody is purified from mouse ascitic fluids by protein-A affinity chromatography .
  • Formulation: It is formulated as a sterile filtered colorless solution containing 1mg/ml PBS (pH 7.4), 10% Glycerol, and 0.02% Sodium Azide .
Applications

The PARK7 antibody has been tested for specificity and reactivity using ELISA and Western blot analysis . It is recommended for use in various laboratory research applications, including:

  • Western Blot Analysis: Recommended dilution range is 1:500 to 2,000, with a starting dilution of 1:1,000 .
  • ELISA: Assures specificity and reactivity .
Storage and Stability

For optimal stability, the PARK7 antibody should be stored at -20°C for up to 12 months or at 4°C for up to 1 month. It is crucial to prevent freeze-thaw cycles to maintain its integrity .

Safety and Usage

ProSpec’s PARK7 antibody products are intended for laboratory research use only and are not suitable for use as drugs, agricultural or pesticidal products, food additives, or household chemicals .

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