PA2G4 Human

Proliferation-associated protein 2G4 Human Recombinant
Cat. No.
BT4355
Source
Escherichia Coli.
Synonyms
Proliferation-associated protein 2G4, Cell cycle protein p38-2G4 homolog, hG4-1, ErbB3-binding protein 1, PA2G4, EBP1, p38-2G4.
Appearance
Sterile filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

PA2G4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 402 amino acids (1-394 a.a.) and having a molecular mass of 44.8kDa. PA2G4 is fused to 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
PA2G4, a member of the peptidase M24C family, is an RNA-binding protein with roles in cellular proliferation and differentiation. It participates in ribosome assembly and rRNA processing as a component of pre-ribosomal ribonucleoprotein complexes. PA2G4 interacts with ErbB-3, potentially modulating Neuregulin-1 signaling in the ErbB-3 pathway. Furthermore, it acts as a transcriptional co-repressor of androgen receptor-regulated and cell cycle regulatory genes by interacting with histone deacetylases. PA2G4 is implicated in growth inhibition and differentiation induction in human cancer cells. Additionally, it mediates cap-independent translation of specific viral internal ribosomal entry sites (IRESs). PA2G4 associates with mature 28S, 18S, and 5.8S rRNAs, various rRNA precursors, and possibly U3 small nucleolar RNA.
Description
PA2G4, produced in E. coli, is a non-glycosylated polypeptide chain containing 402 amino acids (with amino acids 1-394) and a molecular mass of 44.8 kDa. It is expressed with an 8-amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, colorless solution that has been sterilized by filtration.
Formulation
The PA2G4 protein is supplied in a solution containing 20mM Tris-HCl buffer at pH 8.0 and 10% glycerol.
Stability
For short-term storage (up to 4 weeks), store the protein at 4°C. For long-term storage, freeze the protein at -20°C. It is recommended to add a carrier protein such as HSA or BSA (0.1%) for extended storage. Avoid repeated freezing and thawing.
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE analysis.
Synonyms
Proliferation-associated protein 2G4, Cell cycle protein p38-2G4 homolog, hG4-1, ErbB3-binding protein 1, PA2G4, EBP1, p38-2G4.
Source
Escherichia Coli.
Amino Acid Sequence
MSGEDEQQEQ TIAEDLVVTK YKMGGDIANR VLRSLVEASS SGVSVLSLCE KGDAMIMEET GKIFKKEKEM KKGIAFPTSI SVNNCVCHFS PLKSDQDYIL KEGDLVKIDL GVHVDGFIAN VAHTFVVDVA QGTQVTGRKA DVIKAAHLCA EAALRLVKPG NQNTQVTEAW NKVAHSFNCT PIEGMLSHQL KQHVIDGEKT IIQNPTDQQK KDHEKAEFEV HEVYAVDVLV SSGEGKAKDA GQRTTIYKRD PSKQYGLKMK TSRAFFSEVE RRFDAMPFTL RAFEDEKKAR MGVVECAKHE LLQPFNVLYE KEGEFVAQFK FTVLLMPNGP MRITSGPFEP DLYKSEMEVQ DAELKALLQS SASRKTQKKK KKKASKTAEN ATSGETLEEN EAGDLEHHHH HH.

Product Science Overview

Gene and Protein Structure

The PA2G4 gene is located on chromosome 12 and is a protein-coding gene. It encodes an RNA-binding protein that is involved in growth regulation. The protein is present in pre-ribosomal ribonucleoprotein complexes and may be involved in ribosome assembly and the regulation of intermediate and late steps of rRNA processing .

Function and Mechanism

PA2G4 interacts with the cytoplasmic domain of the ErbB3 receptor, contributing to the transduction of growth regulatory signals. It also acts as a transcriptional co-repressor of androgen receptor-regulated genes and other cell cycle regulatory genes through its interactions with histone deacetylases . This protein has been implicated in growth inhibition and the induction of differentiation of human cancer cells .

Expression and Localization

PA2G4 is ubiquitously expressed in various tissues, with cytoplasmic localization. It is involved in several pathways, including the innate immune system and the coregulation of androgen receptor activity . The protein’s expression and localization suggest its involvement in fundamental cellular processes.

Clinical Significance

Mutations or dysregulation of the PA2G4 gene have been associated with several diseases, including trichodentoosseous syndrome and prostate cancer . Its role in growth inhibition and cancer cell differentiation makes it a potential target for therapeutic interventions.

Research and Applications

Human recombinant PA2G4 is used in research to study its function and mechanism in cellular processes. It is also utilized in drug development and cancer research due to its involvement in growth regulation and signal transduction pathways.

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THE BIOTEK
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