OSTF1 Antibody

Osteoclast Stimulating Factor-1 (OSTF1) is an intracellular protein that plays a crucial role in bone resorption by enhancing osteoclast formation and activity. This protein is produced by osteoclasts and is involved in a signaling cascade that results in the secretion of factors promoting osteoclast activity .

OSTF1 is a small intracellular protein containing an SH3 domain followed by four ankyrin domains . It was initially identified as SH3P2 in a screen for Src-homology 3 (SH3)-containing proteins . The protein is known to interact with several intracellular proteins, including F-actin, the non-receptor tyrosine kinase c-Src, and the E3 ubiquitin-protein ligase Casitas B-lineage lymphoma (Cbl) . These interactions are crucial for the bone-resorption properties of osteoclasts .

OSTF1 is widely expressed in the vasculature of most organs and in various cell types in both adult and embryonic mouse tissues . Northern blot analysis has indicated the presence of a single OSTF1 transcript in multiple human tissues .

The primary function of OSTF1 is to induce bone resorption. It acts through a signaling cascade that enhances osteoclast formation and activity . Overexpression of OSTF1 in HeLa cells has been found to negatively impact cell motility, resulting in more rounded cells with smaller footprints .

OSTF1 indirectly enhances osteoclast formation and bone-resorption activity through the supernatant of transfected 293 cells . The protein’s interaction with Cbl in the podosomes of osteoclast-like cells is suggested to be important for their bone-resorption properties .

The regulation of OSTF1 involves its interactions with various intracellular proteins. These interactions are essential for its role in bone resorption and osteoclast activity .