ORM2 Human, sf9

ORM2 is a glycosylated polypeptide chain consisting of 192 amino acids, with a molecular mass of approximately 22.7 kDa . When produced in Sf9 Baculovirus cells, the molecular size on SDS-PAGE appears to be between 28-40 kDa . The recombinant form of ORM2 is fused to a 6-amino acid His-Tag at the C-terminus, which aids in its purification through chromatographic techniques .

ORM2 functions as a transport protein in the bloodstream, binding various hydrophobic ligands within its beta-barrel domain . It also binds synthetic drugs, influencing their distribution and availability. Additionally, ORM2 plays a role in modulating the immune system’s activity during the acute-phase reaction .

The recombinant human ORM2 protein is produced in Sf9 Baculovirus cells. This production method ensures that the protein is glycosylated, which is crucial for its stability and function . The protein is formulated in a phosphate-buffered saline solution (pH 7.4) with 10% glycerol, and it is recommended to store it at 4°C for short-term use or at -20°C for long-term storage .

Recombinant ORM2 is primarily used for laboratory research purposes. It is not intended for use as a drug, agricultural or pesticidal product, food additive, or household chemical . The protein’s high purity (>90%) makes it suitable for various biochemical assays and research applications .

For optimal stability, it is recommended to store ORM2 at 4°C if it will be used within 2-4 weeks. For longer storage periods, freezing at -20°C is advised, with the addition of a carrier protein (0.1% HSA or BSA) to prevent degradation . It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .