ORM1 Human, HEK

ORM1 is a heavily glycosylated serum protein with a molecular mass of approximately 22.4 kDa. The human recombinant version of ORM1 produced in HEK293 cells (Human Embryonic Kidney cells) is a polypeptide chain containing 189 amino acids (19-201) and is fused to a 6 amino acid His tag at the C-terminus . This recombinant protein is purified using proprietary chromatographic techniques to ensure high purity, typically greater than 90% as determined by SDS-PAGE .

ORM1 is an important acute-phase reactant, meaning its levels in the blood increase significantly in response to inflammation, infection, or trauma. It works in conjunction with other acute-phase proteins like haptoglobin and C-reactive protein to modulate the immune response and facilitate the movement of immune cells to sites of infection or injury .

The expression of ORM1 is induced by acute-phase stimulatory mediators such as bacterial lipopolysaccharides. This induction helps the body respond more effectively to bacterial infections by enhancing the immune system’s ability to target and eliminate pathogens .

Recombinant ORM1 produced in HEK293 cells is widely used in laboratory research to study its role in various physiological and pathological processes. It is particularly valuable in research focused on inflammation, immune response, and drug binding studies. The recombinant protein’s high purity and specific structure make it an ideal tool for these applications .

For optimal stability, ORM1 should be stored at 4°C if it will be used within 2-4 weeks. For longer-term storage, it is recommended to store the protein frozen at -20°C, with the addition of a carrier protein such as 0.1% HSA or BSA to prevent degradation. It is important to avoid multiple freeze-thaw cycles to maintain the protein’s integrity .