ODC1 antibody
ODC, Ornithine decarboxylase 1, EC 4.1.1.17.
Description
Product Specs
ODC, Ornithine decarboxylase 1, EC 4.1.1.17.
ODC1 antibody was purified from mouse ascitic fluids by protein-A affinity chromatography.
PAT9B3AT.
Anti-human ODC1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human ODC1 amino acids 1-461 purified from E. coli.
Mouse IgG2b heavy chain and k light chain.
Product Science Overview
Ornithine Decarboxylase 1 (ODC1) is a crucial enzyme in the polyamine biosynthesis pathway. It catalyzes the decarboxylation of ornithine to produce putrescine, which is a precursor for polyamines such as spermidine and spermine. These polyamines are essential for cell proliferation and are involved in various cellular processes, including DNA replication, transcription, and apoptosis .
The ODC1 gene is located on chromosome 2p25 and encodes a protein that is highly conserved across species. The enzyme functions as a homodimer and has a high turnover rate compared to other mammalian proteins . Multiple alternatively spliced transcript variants encoding distinct isoforms have been identified, highlighting the complexity of its regulation and function .
ODC1 is the rate-limiting enzyme in the polyamine biosynthesis pathway. Polyamines are small organic cations that play critical roles in stabilizing DNA structure, modulating ion channels, and regulating gene expression. The activity of ODC1 is tightly regulated by growth-promoting stimuli and is essential for normal cellular function and proliferation .
ODC1 has been implicated in various diseases, including cancer and neurodegenerative disorders. Overexpression of ODC1 is often observed in cancer cells, where it contributes to uncontrolled cell growth and proliferation. Inhibition of ODC1 has been explored as a therapeutic strategy in cancer treatment . Additionally, recent studies have shown that ODC1 plays a role in the astrocytic urea cycle and the putrescine-to-GABA conversion pathway in the brain, which is relevant to Alzheimer’s disease .
Mouse anti-human ODC1 antibodies are monoclonal antibodies developed to specifically target and bind to the human ODC1 protein. These antibodies are commonly used in various research applications, including immunohistochemistry (IHC), immunocytochemistry (ICC), immunofluorescence (IF), and western blotting (WB) . They are valuable tools for studying the expression and function of ODC1 in different biological contexts.
- Immunohistochemistry (IHC): Used to detect ODC1 expression in tissue samples, providing insights into its role in different tissues and disease states.
- Immunocytochemistry (ICC) and Immunofluorescence (IF): Employed to visualize ODC1 localization within cells, aiding in the understanding of its cellular functions.
- Western Blotting (WB): Utilized to quantify ODC1 protein levels in various samples, facilitating studies on its regulation and involvement in different pathways .
