OAT Antibody
Description
Product Specs
Product Science Overview
Ornithine aminotransferase (OAT), also known as ornithine–oxo-acid aminotransferase, is a mitochondrial enzyme that plays a crucial role in the urea cycle and amino acid metabolism. It catalyzes the reversible conversion of ornithine and alpha-ketoglutarate to glutamate semialdehyde and glutamate. This reaction is essential for the proper functioning of the urea cycle, which detoxifies ammonia by converting it into urea for excretion.
OAT is encoded by the OAT gene, which is located on chromosome 10 in humans. The enzyme is composed of 439 amino acids and belongs to the class III pyridoxal-phosphate-dependent aminotransferase family . OAT is typically found as a homotetramer in the mitochondrial matrix, where it performs its catalytic functions.
Deficiency in OAT activity can lead to a rare genetic disorder known as gyrate atrophy of the choroid and retina. This condition is characterized by progressive vision loss due to the degeneration of the choroid and retina. Patients with gyrate atrophy have elevated levels of ornithine in their blood, which is a result of the impaired conversion of ornithine to glutamate semialdehyde .
Mouse anti-human OAT antibodies are monoclonal antibodies produced by immunizing mice with human OAT protein. These antibodies are highly specific to human OAT and are commonly used in various research applications, including Western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry (IHC), and enzyme-linked immunosorbent assay (ELISA) .
One example of a mouse anti-human OAT antibody is the OAT Antibody (A-12) from Santa Cruz Biotechnology. This antibody is an IgG2a κ mouse monoclonal antibody that detects hepatic and renal forms of OAT from mouse, rat, and human origins. It is available in various conjugated forms, including agarose, HRP, PE, FITC, and multiple Alexa Fluor conjugates .
Mouse anti-human OAT antibodies are valuable tools in biomedical research. They are used to study the expression and localization of OAT in different tissues and under various physiological and pathological conditions. These antibodies can help researchers understand the role of OAT in amino acid metabolism, the urea cycle, and related metabolic disorders.
