NUDT16L1 Human

Nudix Type Motif 16 Like-1 Human Recombinant
Cat. No.
BT16596
Source
Escherichia Coli.
Synonyms
Protein syndesmos, NUDT16-like protein 1, NUDT16L1, SDOS, MGC11275.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 95.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NUDT16L1 Human Recombinant fused with a 20 amino acid His tag at N-terminus produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 231 amino acids (1-211 a.a.) and having a molecular mass of 25.5kDa. The NUDT16L1 is purified by proprietary chromatographic techniques.

Product Specs

Introduction
Syndesmos (NUDT16L1), found in the cytoplasm, exhibits specific interaction with the cytoplasmic domain of syndecan-4. This interaction leads to their co-localization within focal contacts. Furthermore, NUDT16L1 interacts with paxillin, a focal adhesion adaptor protein. The interaction between NUDT16L1 and paxillin is direct and is initiated by the activation of protein kinase C.
Description
Recombinant human NUDT16L1, produced in E. coli, is fused with a 20 amino acid His tag at its N-terminus. This results in a single, non-glycosylated polypeptide chain comprising 231 amino acids (amino acids 1-211) with a molecular weight of 25.5kDa. The purification of NUDT16L1 is carried out using proprietary chromatographic techniques.
Physical Appearance
The product is a colorless solution that has undergone sterile filtration.
Formulation
The NUDT16L1 solution is provided at a concentration of 0.5 mg/ml. The solution contains 20mM Tris-HCl buffer with a pH of 8.0, 20% glycerol, 0.1M NaCl, and 1mM DTT.
Stability
For optimal storage, the product should be kept at 4°C if it is expected to be used within 2-4 weeks. For longer storage durations, freezing at -20°C is recommended. To further enhance long-term storage stability, the addition of a carrier protein (0.1% HSA or BSA) is advised. It is important to avoid repeated freeze-thaw cycles to preserve product integrity.
Purity
The purity of NUDT16L1 is determined using SDS-PAGE analysis, which indicates a purity greater than 95.0%.
Synonyms
Protein syndesmos, NUDT16-like protein 1, NUDT16L1, SDOS, MGC11275.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MSTAAVPELK QISRVEAMRL GPGWSHSCHA MLYAANPGQL FGRIPMRFSV LMQMRFDGLL GFPGGFVDRR FWSLEDGLNR VLGLGLGCLR LTEADYLSSH LTEGPHRVVA HLYARQLTLE QLHAVEISAV HSRDHGLEVL GLVRVPLYTQ KDRVGGFPNF LSNAFVSTAK CQLLFALKVL NMMPEEKLVE ALAAATEKQK KALEKLLPAS S.

Product Science Overview

Structure and Expression

NUDT16L1 is a single, non-glycosylated polypeptide chain consisting of 231 amino acids, with a molecular mass of approximately 25.5 kDa . The human recombinant form of NUDT16L1 is produced in Escherichia coli (E. coli) and is fused with a 20 amino acid His tag at the N-terminus to facilitate purification .

Function

The primary function of NUDT16L1 is to hydrolyze nucleoside diphosphates linked to other moieties, which is crucial for maintaining cellular homeostasis. By breaking down potentially harmful nucleotide derivatives, NUDT16L1 helps to prevent the accumulation of these compounds, which could otherwise lead to cellular damage.

Biological Significance

NUDT16L1 plays a significant role in various cellular processes, including:

  • DNA Repair: By hydrolyzing damaged nucleotides, NUDT16L1 helps to maintain the integrity of the genetic material.
  • Signal Transduction: The enzyme’s activity can influence various signaling pathways by modulating the levels of nucleotide derivatives.
  • Metabolic Regulation: NUDT16L1 is involved in the regulation of metabolic processes by controlling the concentration of nucleotides and their derivatives.
Research and Applications

The recombinant form of NUDT16L1 is widely used in research to study its enzymatic activity and its role in cellular processes. It is also used to investigate potential therapeutic applications, such as targeting NUDT16L1 for the treatment of diseases associated with nucleotide metabolism disorders.

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NUDT16 Human
Nudix Type Motif 16 Human Recombinant
Cat. No.
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Source
Escherichia Coli.
THE BIOTEK
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