NTS Human, sf9

Neurotensin Human Recombinant, sf9
Cat. No.
BT1625
Source
Sf9, Baculovirus cells.
Synonyms
Neurotensin/neuromedin N, NTS, Neuromedin N, NN, NmN, NT, NmN-125.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90.0% as determined by analysis by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description

NTS Human Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 134 amino acids (24-148) and having a molecular mass of 15.4kDa (Molecular size on SDS-PAGE will appear at approximately 13.5-18kDa).
NTS is fused to 6 amino acid His-Tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specs

Introduction
Neurotensin (NTS), a precursor to neuromedin N and neurotensin peptides, is a tridecapeptide found throughout the central nervous system. It is believed to function as a neurotransmitter or neuromodulator. NTS plays a role in dopamine-related processes, gut health, and fat metabolism. In certain tissues, larger forms of neuromedin N and neurotensin may be produced, potentially offering enhanced stability and bioactivity.
Description
Recombinant Human NTS, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain with a molecular weight of 15.4kDa (134 amino acids, 24-148). SDS-PAGE analysis may show a molecular size of approximately 13.5-18kDa. The NTS protein is fused to a 6 amino acid His-Tag at the C-terminus and purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile-filtered solution.
Formulation
The NTS protein solution (1mg/ml) is supplied in Phosphate Buffered Saline (pH 7.4) with 10% glycerol.
Stability
For short-term storage (2-4 weeks), keep at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90.0% as determined by SDS-PAGE analysis.
Synonyms
Neurotensin/neuromedin N, NTS, Neuromedin N, NN, NmN, NT, NmN-125.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
ADPSDSEEEM KALEADFLTN MHTSKISKAH VPSWKMTLLN VCSLVNNLNS PAEETGEVHE EELVARRKLP TALDGFSLEA MLTIYQLHKI CHSRAFQHWE LIQEDILDTG NDKNGKEEVI KRKIPYILHH HHHH.

Product Science Overview

Physiological Functions

Neurotensin has a variety of physiological roles:

  • In the CNS: It is involved in the regulation of dopamine pathways, which has implications for conditions such as schizophrenia and Parkinson’s disease . Neurotensin also exhibits opioid-independent analgesic properties .
  • In the gastrointestinal tract: It increases intestinal motility, stimulates pancreatic and biliary secretions, and promotes the growth of tissues such as the gut, pancreas, adrenal gland, and liver .
Receptors

Neurotensin exerts its effects through three main receptors:

  1. Neurotensin receptor 1 (NTSR1): A seven-transmembrane G-protein coupled receptor that has a high affinity for neurotensin .
  2. Neurotensin receptor 2 (NTSR2): Another seven-transmembrane G-protein coupled receptor .
  3. Neurotensin receptor 3 (NTSR3): Also known as sortilin 1, this is a single transmembrane domain sorting receptor .
Role in Cancer

Neurotensin and its receptors have been implicated in the proliferation, migration, and DNA synthesis of tumor cells . The NT/NTSR1 complex, in particular, activates phospholipase C (PLC), leading to the production of inositol triphosphate (IP3) and diacylglycerol (DAG) from membrane phospholipids . This signaling pathway is crucial for various cellular processes, including those involved in cancer progression .

Recombinant Neurotensin (Human, sf9)

Recombinant neurotensin is produced using various expression systems, including the sf9 insect cell line. This recombinant form is often tagged with a His-tag for purification purposes and is used in research to study the peptide’s functions and interactions . The recombinant protein is typically expressed in E. coli and purified to a high degree of purity .

Applications

Recombinant neurotensin is used in various research applications, including:

  • Functional studies: To understand its role in neurotransmission and neuromodulation.
  • Cancer research: To study its involvement in tumor growth and metastasis.
  • Drug development: As a potential target for therapeutic interventions in conditions like schizophrenia, Parkinson’s disease, and cancer .

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