NTS Human

Neurotensin Human Recombinant
Cat. No.
BT1557
Source
Escherichia Coli.
Synonyms
NTS, Neurotensin, NT/N, NMN-125, NTS1, NN, Neurotensin/Neuromedin N, NT.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
Shipped with Ice Packs
In Stock
Quick Inquiry

Description

NTS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 172 amino acids (24-170 a.a.) and having a molecular mass of 19.9kDa.
NTS is fused to a 25 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.

Product Specs

Introduction
Neurotensin (NTS) is a precursor for two peptides: neuromedin N and neurotensin. Neurotensin, a secreted tridecapeptide found throughout the central nervous system, may act as a neurotransmitter or neuromodulator. NTS contributes to maintaining gut structure and function and regulating fat metabolism. In certain tissues, tissue-specific processing might lead to the formation of larger, potentially more stable and biologically active forms of neuromedin N and neurotensin.
Description
Recombinant human NTS, produced in E. coli, is a single, non-glycosylated polypeptide chain comprising 172 amino acids (24-170 a.a.) with a molecular weight of 19.9 kDa. It features a 25 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
A clear, sterile solution.
Formulation
The NTS protein solution (1 mg/ml) is supplied in 20 mM Tris-HCl buffer (pH 8.0), containing 0.4 M urea and 10% glycerol.
Stability
For short-term use (2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. Adding a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
NTS, Neurotensin, NT/N, NMN-125, NTS1, NN, Neurotensin/Neuromedin N, NT.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSDSEE EMKALEADFL TNMHTSKISK AHVPSWKMTL LNVCSLVNNL NSPAEETGEV HEEELVARRK LPTALDGFSL EAMLTIYQLH KICHSRAFQH WELIQEDILD TGNDKNGKEE VIKRKIPYIL KRQLYENKPR RPYILKRDSY YY.

Product Science Overview

Introduction

Neurotensin is a tridecapeptide (13 amino acids) neuropeptide that was first isolated in 1973. It is widely distributed throughout the central nervous system (CNS) and the gastrointestinal tract. Neurotensin functions as both a neurotransmitter and a neuromodulator, playing a crucial role in various physiological processes.

Structure and Synthesis

Neurotensin is derived from a larger precursor protein known as pro-neurotensin/neuromedin N. The human neurotensin gene (NTS) encodes this precursor, which is subsequently cleaved to produce neurotensin and another peptide, neuromedin N . The recombinant form of human neurotensin is typically produced in E. coli or HEK293 cells, with the protein often tagged for purification purposes .

Biological Functions

Neurotensin has a wide range of biological functions, including:

  • Neuromodulation: It modulates dopamine signaling, which is implicated in various neurological and psychiatric disorders.
  • Endocrine Regulation: Neurotensin influences the release of several hormones, including insulin and glucagon.
  • Gastrointestinal Function: It plays a role in the maintenance of gut structure and function, including the regulation of intestinal motility and secretion.
  • Pain Modulation: Neurotensin has been shown to have analgesic properties, making it a potential target for pain management therapies .
Receptors and Mechanism of Action

Neurotensin exerts its effects through three main receptors: NTS1, NTS2, and NTS3. These receptors are G-protein coupled receptors (GPCRs) that activate various intracellular signaling pathways. NTS1 is primarily involved in the modulation of dopamine pathways, while NTS2 and NTS3 have roles in pain modulation and neuroprotection .

Clinical Implications

Neurotensin and its receptors have been implicated in several diseases, including:

  • Cancer: Neurotensin promotes tumor cell proliferation, migration, and DNA synthesis in various cancers .
  • Neurological Disorders: Dysregulation of neurotensin signaling is associated with schizophrenia, Parkinson’s disease, and other neurological conditions.
  • Metabolic Disorders: Neurotensin influences fat metabolism and has been linked to obesity and type 2 diabetes .

Quick Inquiry

Personal Email Detected
Please use an institutional or corporate email address for inquiries. Personal email accounts ( such as Gmail, Yahoo, and Outlook) are not accepted. *

Category

Service

Related Products

NTS Human, sf9
Neurotensin Human Recombinant, sf9
Cat. No.
BT1625
Source
Sf9, Baculovirus cells.
THE BIOTEK
THE BioTek is a global biotech company offering highly purified proteins for research in cancer, apoptosis, development, endocrinology, immunology, neuroscience, proteases, and stem cells.
  • Contact
  • Address: 1925 E Maple Ave, El Segundo, CA 90245 United States
  • Phone : +1 201-285-7826
  • Email : info@thebiotek.com
  • Hours : Mon.-Fri. 9:00 AM - 5:00 PM
© Copyright 2024 Thebiotek. All Rights Reserved.