NTRK2 Human , HEK
HEK293 Cells.
GP145-TrkB, trk-B, TRKB, NTRK2.
Sterile Filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
NTRK2 Human Recombinant is a single, glycosylated, polypeptide chain (32-430 a.a) containing a total of 632 amino acids and having a molecular mass of 70.3 kDa.
NTRK2 is fused to a 233 a.a hIgG-His-Tag at C-terminus and is purified by proprietary chromatographic techniques.
Product Specs
Recombinant human NTRK2 is a single polypeptide chain with glycosylation. It encompasses amino acids 32 to 430, resulting in a total of 632 amino acids. The molecular mass is 70.3 kDa. This protein is fused to a 233 amino acid hIgG-His-Tag at its C-terminus. Purification is achieved through proprietary chromatographic methods.
A colorless solution that has been sterilized by filtration.
The NTRK2 solution is provided at a concentration of 1 mg/ml. It is formulated in a solution of Phosphate-Buffered Saline (pH 7.4) containing 10% Glycerol.
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, it should be frozen at -20°C. To ensure stability during long-term storage, the addition of a carrier protein (0.1% HSA or BSA) is recommended. Repeated freezing and thawing should be avoided.
The purity of this product, as determined by SDS-PAGE, is greater than 90%.
GP145-TrkB, trk-B, TRKB, NTRK2.
HEK293 Cells.
CPTSCKCSAS RIWCSDPSPG IVAFPRLEPN SVDPENITEI FIANQKRLEI INEDDVEAYV GLRNLTIVDS GLKFVAHKAF LKNSNLQHIN FTRNKLTSLS RKHFRHLDLS ELILVGNPFT CSCDIMWIKT LQEAKSSPDT QDLYCLNESS KNIPLANLQI PNCGLPSANL AAPNLTVEEG KSITLSCSVA GDPVPNMYWD VGNLVSKHMN ETSHTQGSLR ITNISSDDSG KQISCVAENL VGEDQDSVNL TVHFAPTITF LESPTSDHHW CIPFTVKGNP KPALQWFYNG AILNESKYIC TKIHVTNHTE YHGCLQLDNP THMNNGDYTL IAKNEYGKDE KQISAHFMGW PGIDDGANPN YPDVIYEDYG TAANDIGDTT NRSNEIPSTD VTDKTGREH LEPKSCDKTHT CPPCPAPELL GGPSVFLFPP KPKDTLMISR TPEVTCVVVD VSHEDPEVKF NWYVDGVEVH NAKTKPREEQ YNSTYRVVSV LTVLHQDWLN GKEYKCKVSN KALPAPIEKT ISKAKGQPRE PQVYTLPPSR DELTKNQVSL TCLVKGFYPS DIAVEWESNG QPENNYKTTP PVLDSDGSFF LYSKLTVDKS RWQQGNVFSC SVMHEALHNH YTQKSLSLSP GK.
Product Science Overview
Neurotrophic Receptor Tyrosine Kinase 2 (NTRK2), also known as tropomyosin-related kinase B (TrkB), is a member of the neurotrophic tyrosine receptor kinase (NTRK) family. This kinase is a membrane-bound receptor that plays a crucial role in the development and function of the nervous system. The recombinant form of NTRK2, expressed in Human Embryonic Kidney (HEK) 293 cells, is widely used in research to study its biological functions and therapeutic potential.
NTRK2 is activated by binding to neurotrophins such as brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4/5 (NT-4/5) . Upon activation, NTRK2 undergoes autophosphorylation and initiates several downstream signaling pathways, including the MAPK pathway, leading to various cellular responses such as:
Abnormal expression of the NTRK2 gene is associated with several cancers, including colorectal, breast, papillary thyroid cancer, lung adenocarcinoma, and oral squamous cell carcinoma . Due to its role in cancer and nervous system development, NTRK2 is a target for therapeutic interventions and drug development.
The recombinant form of NTRK2 is expressed in HEK 293 cells, a widely used cell line derived from human embryonic kidney cells. This recombinant protein is typically tagged with a 6-His tag at the C-terminus and is purified to a high degree of purity (≥95% as determined by SDS-PAGE) . The recombinant NTRK2 protein is used in various biochemical and physiological studies to understand its function and to develop potential therapeutic agents.
