NTRK2 Human
Sf9, Baculovirus cells.
GP145-TrkB, trk-B, TRKB, NTRK2
Greater than 95% as determined by SDS-PAGE.
Description
NTRK2 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 407 amino acids (32-430a.a) and having a molecular mass of 45.2kDa.
NTRK2 is fused to an 8 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
Neurotrophic Receptor Tyrosine Kinase 2, also known as NTRK2, is a tyrosine-protein kinase receptor involved in the development and maturation of the central and peripheral nervous systems. It regulates neuronal survival, migration, proliferation, differentiation, and synapse formation and plasticity. NTRK2 plays a role in learning and memory by regulating both short-term synaptic function and long-term potentiation. Known substrates for TRK family receptors include SHC1, PI-3 kinase, and PLC-gamma-1.
Human NTRK2, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain containing 407 amino acids (32-430a.a). It has a molecular mass of 45.2kDa. NTRK2 is fused to an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic techniques.
The NTRK2 solution is supplied at a concentration of 0.5mg/ml and contains 10% glycerol in phosphate-buffered saline (pH 7.4).
For short-term storage (2-4 weeks), the product can be stored at 4°C. For long-term storage, it is recommended to store the product frozen at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity is determined to be greater than 95% by SDS-PAGE analysis.
GP145-TrkB, trk-B, TRKB, NTRK2
Sf9, Baculovirus cells.
CPTSCKCSAS RIWCSDPSPG IVAFPRLEPN SVDPENITEI FIANQKRLEI INEDDVEAYV GLRNLTIVDS GLKFVAHKAF LKNSNLQHIN FTRNKLTSLS RKHFRHLDLS ELILVGNPFT CSCDIMWIKT LQEAKSSPDT QDLYCLNESS KNIPLANLQI PNCGLPSANL AAPNLTVEEG KSITLSCSVA GDPVPNMYWD VGNLVSKHMN ETSHTQGSLR ITNISSDDSG KQISCVAENL VGEDQDSVNL TVHFAPTITF LESPTSDHHW CIPFTVKGNP KPALQWFYNG AILNESKYIC TKIHVTNHTE YHGCLQLDNP THMNNGDYTL IAKNEYGKDE KQISAHFMGW PGIDDGANPN YPDVIYEDYG TAANDIGDTT NRSNEIPSTD VTDKTGREHL EHHHHHH
Product Science Overview
Neurotrophic Receptor Tyrosine Kinase 2 (NTRK2), also known as TrkB, is a critical protein in the development and maintenance of the mammalian nervous system. It belongs to the neurotrophic factor family of related polypeptides and serves as a receptor for brain-derived neurotrophic factor (BDNF) and neurotrophin-4 (NTF4). NTRK2 plays a pivotal role in neuron survival, proliferation, migration, differentiation, and synapse formation and plasticity .
NTRK2 is a membrane-bound receptor tyrosine kinase. Upon binding with its ligands, such as BDNF, it undergoes homodimerization, autophosphorylation, and activation. This activation triggers downstream signaling pathways, including the MAPK pathway, which leads to various cellular responses essential for nervous system development and function .
NTRK2 is predominantly expressed in the brain, with enhanced expression in regions such as the cerebral cortex, cerebellum, basal ganglia, hypothalamus, midbrain, amygdala, choroid plexus, hippocampal formation, and spinal cord. It is also found in the thyroid gland and other tissues. The protein is localized mainly to the plasma membrane and intracellular compartments .
Mutations and abnormal expression of the NTRK2 gene are associated with several neurological and psychiatric disorders, including obesity, mood disorders, and developmental and epileptic encephalopathy. Additionally, NTRK2 has been implicated in various cancers, such as colorectal, breast, papillary thyroid cancer, lung adenocarcinoma, and oral squamous cell carcinoma .
Recombinant NTRK2 proteins are widely used in research to study their role in neurobiology and disease. These proteins are also being explored as potential therapeutic targets for treating neurological disorders and cancers. The development of NTRK2 inhibitors and other modulators holds promise for future therapeutic interventions .
