NTRK1 Rat
TRKA, TRK-A, NTRK-1.
Greater than 90.0% as determined by SDS-PAGE.
Description
NTRK1 Rat Recombinant produced in HEK is a single, non-glycosylated polypeptide chain containing 623 amino acids (35-418 a.a) and having a molecular mass of 69 kDa. NTRK1 is fused to a 239 amino acid human IgG-His-tag at C-terminus & purified by proprietary chromatographic techniques.
Product Specs
NTRK1, a key player in the development and survival of nerve cells (neurons), is especially crucial for sensory neurons that transmit data related to touch, pain, and temperature. This protein, primarily found on the surface of cells like sensory neurons, acts as a kinase enzyme. It modifies the activity of various proteins by adding a phosphate group along with oxygen atoms at specific sites, a process known as phosphorylation. NTRK1 is activated upon binding with nerve growth factor beta, which triggers the protein to phosphorylate itself (autophosphorylation). This activated NTRK1 then phosphorylates other proteins, a crucial step for cell growth and survival.
Recombinant NTRK1 protein, produced in HEK cells from Rat, is a single, non-glycosylated polypeptide chain. This protein consists of 623 amino acids (specifically, amino acids 35 to 418) and has a molecular weight of 69 kDa. It is fused to a 239 amino acid long human IgG-His-tag at its C-terminus and is purified using proprietary chromatographic techniques.
The NTRK1 protein is supplied as a solution with a concentration of 1mg/ml. This solution is prepared in Phosphate-Buffered Saline (pH 7.4) and contains 10% Glycerol.
While NTRK1 remains stable for up to 1 week at a refrigerated temperature of 4°C, it is recommended to store it at a temperature below -18°C for optimal long-term storage. To further enhance its stability during long-term storage, consider adding a carrier protein like HSA or BSA at a concentration of 0.1%. It is important to avoid repeated freeze-thaw cycles to maintain the protein's integrity.
The biological activity of this protein is assessed based on its ability to inhibit NGF-induced proliferation in TF1 human erythroleukemic cells. This assay is carried out in the presence of 0.5 ng/ml of rat NGF. The ED50 for this inhibition is found to be within the range of ≤ 5 ng/ml.
Analysis using SDS-PAGE indicates that the purity of this protein is greater than 90%.
TRKA, TRK-A, NTRK-1.
SCRETCCPVG PSGLRCTRAG TLNTLRGLRG AGNLTELYVE NQRDLQRLEF EDLQGLGELR SLTIVKSGLR FVAPDAFHFT PRLSHLNLSS NALESLSWKT VQGLSLQDLT LSGNPLHCSC ALLWLQRWEQ EDLCGVYTQK LQGSGSGDQF LPLGHNNSCG VPSVKIQMPN DSVEVGDDVF LQCQVEGQAL QQADWILTEL EGTATMKKSG DLPSLGLTLV NVTSDLNKKN VTCWAENDVG RAEVSVQVSV SFPASVHLGK AVEQHHWCIP FSVDGQPAPS LRWFFNGSVL NETSFIFTQF LESALTNETM RHGCLRLNQP THVNNGNYTL LAANPYGQAA ASIMAAFMDN PFEFNPEDPI PVSFSPVDTN STSRDPVEKK DETP LEPKSC DKTHTCPPCP APELLGGPSV FLFPPKPKDT LMISRTPEVT CVVVDVSHED PEVKFNWYVD GVEVHNAKTK PREEQYNSTY RVVSVLTVLH QDWLNGKEYK CKVSNKALPA PIEKTISKAK GQPREPQVYT LPPSRDELTK NQVSLTCLVK GFYPSDIAVE WESNGQPENN YKTTPPVLDS DGSFFLYSKL TVDKSRWQQG NVFSCSVMHE ALHNHYTQKS LSLSPGKHHH HHH
Product Science Overview
Neurotrophic Receptor Tyrosine Kinase 1 (NTRK1), also known as Tropomyosin Receptor Kinase A (TrkA), is a protein encoded by the NTRK1 gene. This receptor is a member of the neurotrophic tyrosine kinase receptor (NTKR) family and plays a crucial role in the development and function of the nervous system . NTRK1 is primarily known for its high affinity binding to Nerve Growth Factor (NGF), which is essential for the survival, differentiation, and maintenance of neurons .
NTRK1 is a membrane-bound receptor that, upon binding with its ligand NGF, undergoes autophosphorylation. This phosphorylation activates several downstream signaling pathways, including the MAPK pathway, which is involved in cell differentiation and survival . The receptor is crucial for the development of sensory neurons and plays a significant role in specifying sensory neuron subtypes .
The NTRK1 receptor is essential for the development and survival of nerve cells, particularly sensory neurons that transmit information about sensations such as pain, temperature, and touch . Mutations in the NTRK1 gene can lead to various disorders, including congenital insensitivity to pain with anhidrosis (CIPA) and certain types of cancer, such as papillary thyroid carcinoma .
Recombinant NTRK1, particularly from rat models, is widely used in research to study its function and role in various biological processes. The recombinant form allows for detailed analysis of the receptor’s structure, function, and interactions with other proteins. It is also used to investigate the effects of mutations and to develop potential therapeutic interventions for related disorders.
