PGK1 Mouse
Phosphoglycerate Kinase 1 Mouse Recombinant
Cat. No.
BT12981
Source
Escherichia Coli.
Synonyms
Phosphoglycerate kinase 1 (EC:2.7.2.3), Pgk1, Pgk-1.
Appearance
Sterile Filtered clear solution.
Purity
Greater than 90% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
PGK1 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 441 amino acids (1-417 a.a) and having a molecular mass of 47.1kDa.
PGK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
PGK1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Introduction
PGK1, an enzyme encoded by the X-linked PGK1 gene, plays a crucial role in glycolysis. This enzyme catalyzes the conversion of 1,3-diphosphoglycerate to 3-phosphoglycerate, producing ATP. Beyond its glycolytic function, PGK1 may also act as a cofactor for polymerase alpha. Defects in the PGK1 gene can lead to chronic hemolytic anemia, sometimes accompanied by mental retardation or muscular disease (rhabdomyolysis). Overexpression of PGK1 and its associated signaling targets may be linked to peritoneal dissemination in diffuse primary gastric carcinomas. Additionally, studies indicate differential expression of PGK1 in the dorsolateral prefrontal cortex of individuals with schizophrenia.
Description
Recombinant PGK1 Mouse protein, expressed in E. coli, is a single, non-glycosylated polypeptide chain. It consists of 441 amino acids (amino acids 1-417) with a molecular weight of 47.1 kDa. The protein includes a 24 amino acid His-tag at the N-terminus and is purified using proprietary chromatographic techniques.
Physical Appearance
Clear solution, sterile filtered.
Formulation
The PGK1 protein solution (1 mg/ml) is supplied in phosphate-buffered saline (pH 7.4) containing 10% glycerol and 1 mM DTT.
Stability
For short-term storage (2-4 weeks), the product can be stored at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity
Purity exceeds 90% as determined by SDS-PAGE analysis.
Biological Activity
The specific activity of the protein is > 500 units/mg, where one unit is defined as the amount of enzyme required to convert 1 micromole of 1,3-Bisphosphoglycerate to 3-PGA per minute at pH 8.0 and 37°C.
Synonyms
Phosphoglycerate kinase 1 (EC:2.7.2.3), Pgk1, Pgk-1.
Source
Escherichia Coli.
Amino Acid Sequence
MGSSHHHHHH SSGLVPRGSH MGSHMSLSNK LTLDKLDVKG KRVVMRVDFN VPMKNNQITN NQRIKAAVPS IKFCLDNGAK SVVLMSHLGR PDGVPMPDKY SLEPVAAELK SLLGKDVLFL KDCVGPEVEN ACANPAAGTV ILLENLRFHV EEEGKGKDAS GNKVKAEPAK IDAFRASLSK LGDVYVNDAF GTAHRAHSSM VGVNLPQKAG GFLMKKELNY FAKALESPER PFLAILGGAK VADKIQLINN MLDKVNEMII GGGMAFTFLK VLNNMEIGTS LYDEEGAKIV KDLMSKAEKN GVKITLPVDF VTADKFDENA KTGQATVASG IPAGWMGLDC GTESSKKYAE AVGRAKQIVW NGPVGVFEWE AFARGTKSLM DEVVKATSRG CITIIGGGDT ATCCAKWNTE DKVSHVSTGG GASLELLEGK VLPGVDALSN V.
Product Science Overview
Structure and Expression
The recombinant mouse PGK1 protein is typically expressed in Escherichia coli and is available in a full-length form, ranging from amino acids 1 to 417 . It is often tagged with a His tag at the N-terminus to facilitate purification and detection. The protein is purified to over 90% purity and is suitable for various applications, including SDS-PAGE and mass spectrometry .
Biological Functions
PGK1 is not only a key player in glycolysis but also exhibits several other biological activities:
- Angiogenesis: PGK1 is secreted by tumor cells and participates in angiogenesis by reducing disulfide bonds in the serine protease plasmin, leading to the release of angiostatin, a tumor blood vessel inhibitor .
- DNA Replication and Repair: PGK1 acts as a cofactor for polymerase alpha, playing a role in DNA replication and repair .
- Tumorigenesis: PGK1 is involved in various processes related to tumorigenesis, including epithelial-mesenchymal transition (EMT), autophagy initiation, and mitochondrial metabolism .
Clinical Significance
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