NRP1 Rat
Neuropilin 1 Rat Recombinant
Cat. No.
BT10914
Source
Sf9, Baculovirus cells.
Synonyms
Neuropilin-1, Vascular endothelial cell growth factor 165 receptor, CD304, Nrp1.
Appearance
Sterile Filtered colorless solution.
Purity
Greater than 90.0% as determined by SDS-PAGE.
Usage
THE BioTek's products are furnished for LABORATORY RESEARCH USE ONLY. The product may not be used as drugs, agricultural or pesticidal products, food additives or household chemicals.
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Description
NRP1 Rat Recombinant produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 842 amino acids (22-855 a.a.) and having a molecular mass of 94.8kDa (Migrates at 100-150kDa on SDS-PAGE under reducing conditions).
NRP1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
NRP1 is expressed with an 8 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.
Product Specs
Introduction
Neuropilin 1 (Nrp1) is a transmembrane glycoprotein that acts as a co-receptor for various extracellular ligands. These ligands include class III/IV semaphorins, specific isoforms of vascular endothelial growth factor (VEGF), and transforming growth factor beta. Nrp1 binds to VEGF-A and is thought to function as a coreceptor for kinase insert domain-containing receptor (KDR). It achieves this by interacting with KDR and amplifying VEGF signaling. Notably, Nrp1 serves as a marker for regulatory T cells.
Description
Recombinant Rat NRP1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain. It comprises 842 amino acids (22-855 a.a.) and has a molecular weight of 94.8kDa. Under reducing conditions on SDS-PAGE, it migrates at 100-150kDa. The protein is expressed with an 8 amino acid His tag at the C-terminus and purified using proprietary chromatographic methods.
Physical Appearance
A sterile, colorless solution.
Formulation
The NRP1 protein solution is provided at a concentration of 0.25mg/ml. It is prepared in Phosphate Buffered Saline (pH 7.4) and contains 10% glycerol.
Stability
For short-term storage (2-4 weeks), the product should be kept at 4°C. For extended storage, it is recommended to freeze the product at -20°C. To ensure long-term stability, adding a carrier protein (0.1% HSA or BSA) is advisable. Avoid repeated freeze-thaw cycles.
Purity
The purity of the NRP1 protein is determined to be greater than 90.0% by SDS-PAGE analysis.
Synonyms
Neuropilin-1, Vascular endothelial cell growth factor 165 receptor, CD304, Nrp1.
Source
Sf9, Baculovirus cells.
Amino Acid Sequence
FRSDKCGGTI KIENPGYLTS PGYPHSYHPS EKCEWLIQAP EPYQRIMINF NPHFDLEDRD CKYDYVEVID GENEGGRLWG KFCGKIAPSP VVSSGPFLFI KFVSDYETHG AGFSIRYEIF KRGPECSQNY TAPTGVIKSP GFPEKYPNSL ECTYIIFAPK MSEIILEFES FDLEQDSNPP GGVFCRYDRL EIWDGFPEVG PHIGRYCGQK TPGRIRSSSG ILSMVFYTDS AIAKEGFSAN YSVLQSSISE DFKCMEALGM ESGEIHSDQI TASSQYGTNW SVERSRLNYP ENGWTPGEDS YREWIQVDLG LLRFVTAVGT QGAISKETKK KYYVKTYRVD ISSNGEDWIT LKEGNKAIIF QGNTNPTDVV FGVFPKPLIT RFVRIKPASW ETGISMRFEV YGCKITDYPC SGMLGMVSGL ISDSQITASN QGDRNWMPEN IRLVTSRTGW ALPPSPHPYI NEWLQVDLGD EKIVRGVIIQ GGKHRENKVF MRKFKIAYSN NGSDWKMIMD DSKRKAKSFE GNNNYDTPEL RAFTPLSTRF IRIYPERATH SGLGLRMELL GCEVEVPTAG PTTPNGNPVD ECDDDQANCH SGTGDDFQLT GGTTVLATEK PTIIDSTIQS EFPTYGFNCE FGWGSHKTFC HWEHDSHAQL RWRVLTSKTG PIQDHTGDGN FIYSQADENQ KGKVARLVSP VVYSQSSAHC MTFWYHMSGS HVGTLRVKLH YQKPEEYDQL VWMVVGHQGD HWKEGRVLLH KSLKLYQVIF EGEIGKGNLG GIAVDDISIN NHIPQEDCAK PTDLDKKNTE IKIDETGSTP GYEEGKGDKN ISRKPGNVLK TLDPLEHHHH HH
Product Science Overview
Structure and Function
Neuropilin-1 consists of several distinct domains:
- Extracellular Domain (ECD): This domain contains two N-terminal CUB domains, two F5/8 type C domains with homology to coagulation factors V and VIII, and a MAM (meprin) domain . The ECD is responsible for binding to various ligands, including vascular endothelial growth factor (VEGF) and semaphorins, which are critical for its role in angiogenesis and axon guidance .
- Transmembrane Domain ™: This domain anchors the protein to the cell membrane.
- Cytoplasmic Domain: Although relatively short, this domain is essential for intracellular signaling.
Recombinant Rat Neuropilin-1
Applications
Recombinant Neuropilin-1 is widely used in research to study its role in:
- Angiogenesis: By binding to VEGF, Neuropilin-1 promotes the formation of new blood vessels, which is crucial in both normal physiological processes and pathological conditions like cancer .
- Axon Guidance: Neuropilin-1 interacts with semaphorins to guide the growth of axons during neural development .
- Cancer Research: Due to its role in angiogenesis, Neuropilin-1 is a target for cancer therapy research. Inhibiting its interaction with VEGF can potentially reduce tumor growth and metastasis .
Stability and Storage
Recombinant Neuropilin-1 is typically lyophilized and should be reconstituted in sterile PBS. It is stable for up to 12 months when stored at -20 to -70°C and should be handled to avoid repeated freeze-thaw cycles .
Neuropilin-1’s multifaceted roles in critical biological processes make it a significant protein for research in developmental biology, neurobiology, and oncology.
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