NRG1 Human, SF9
Sf9, Baculovirus cells.
Neuregulin-1, NRG1, GGF, HGL, HRGA, NDF, SMDF, HRG, ARIA, GGF2, HRG1
Sterile filtered colorless solution.
Greater than 90.0% as determined by SDS-PAGE.
Description
NRG1 Human produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 231 amino acids (20-241 aa) and having a molecular mass of 25.1kDa.
NRG1 is fused to a 6 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.
Product Specs
The Neuregulin/Heregulin family consists of structurally similar polypeptide growth factors originating from alternatively spliced genes (NRG1, NRG2, NRG3, and NRG4). The NRG1 gene alone gives rise to over 14 soluble and transmembrane proteins. Soluble growth factors are produced through the proteolytic cleavage of the extracellular domain of transmembrane NRG1 isoforms. These isoforms encompass heregulins (HRGs), glial growth factors (GGFs), and sensory and motor neuron-derived factor (SMDF). Sharing common Ig and EGF-like domains, these factors bind to ErbB3 and ErbB4 receptor tyrosine kinases, inducing their heterodimerization with ErbB2. This interaction activates intrinsic kinase activity, leading to tyrosine phosphorylation. NRG1 isoforms play a crucial role in the growth and differentiation of various cell types, including epithelial, neuronal, and glial cells.
Human NRG1, produced in Sf9 Baculovirus cells, is a single, glycosylated polypeptide chain consisting of 231 amino acids (20-241 aa). It has a molecular weight of 25.1 kDa. A 6-amino acid His tag is fused to the C-terminus of NRG1. The protein is purified using proprietary chromatographic techniques.
A sterile, colorless solution.
The NRG1 solution is provided at a concentration of 0.5 mg/ml in a buffer containing 10% glycerol and phosphate-buffered saline (pH 7.4).
For short-term storage (up to 2-4 weeks), store at 4°C. For extended storage, freeze at -20°C. The addition of a carrier protein (0.1% HSA or BSA) is recommended for long-term storage. Avoid repeated freeze-thaw cycles.
Purity is determined to be greater than 90.0% by SDS-PAGE analysis.
Biological activity is assessed through a cell proliferation assay using MCF7 human breast cancer cells. The ED50 for this effect is less than or equal to 15 ng/ml.
Neuregulin-1, NRG1, GGF, HGL, HRGA, NDF, SMDF, HRG, ARIA, GGF2, HRG1
Sf9, Baculovirus cells.
ADPSGKKPES AAGSQSPALP PRLKEMKSQE SAAGSKLVLR CETSSEYSSL RFKWFKNGNE
LNRKNKPQNI KIQKKPGKSE LRINKASLAD SGEYMCKVIS KLGNDSASAN ITIVESNEII TGMPASTEGA YVSSESPIRI SVSTEGANTS SSTSTSTTGT SHLVKCAEKE KTFCVNGGEC FMVKDLSNPS RYLCKCPNEF TGDRCQNYVM ASFYSTSTPF LSLPEHHHHH H
Product Science Overview
NRG1 is encoded by the NRG1 gene, which produces more than 14 different isoforms through alternative splicing. These isoforms can be broadly categorized into soluble and transmembrane proteins. The transmembrane isoforms undergo proteolytic processing to release soluble growth factors, which include heregulins (HRGs), glial growth factors (GGFs), and sensory and motor neuron-derived factor (SMDF) .
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Cardiovascular System: NRG1 is a potent cardiomyocyte proliferator, secreted and released by endothelial vascular cells. It plays a major role in heart growth, proliferation, differentiation, apoptosis, and other cardiovascular processes. NRG1 can repair the heart in conditions such as atherosclerosis, myocardial infarction, ischemia-reperfusion injury, heart failure, and cardiomyopathy . It activates multiple signaling pathways, including the NRG1/ErbB pathway, to improve the myocardial microenvironment by regulating cardiac inflammation, oxidative stress, and necrotic apoptosis .
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Nervous System: NRG1 is also crucial for neural development and function. It regulates inhibitory neural processes important for executive functions such as attention and working memory. Variants in the NRG1 gene have been associated with neurodevelopmental disorders like schizophrenia and attention deficit hyperactivity disorder (ADHD) . NRG1 activates signaling cascades that are involved in learning, memory, and other higher brain functions .
Given its significant roles in both the cardiovascular and nervous systems, NRG1 has been explored as a therapeutic target for various conditions. In the cardiovascular field, NRG1 has shown promise in promoting myocardial repair and improving cardiac function . In the context of neurodevelopmental disorders, modulating NRG1 signaling pathways could potentially reactivate cortical plasticity and improve cognitive outcomes .
Human recombinant NRG1 produced in Sf9 cells (a type of insect cell line) is used in research and therapeutic applications. The recombinant form ensures a consistent and high-quality supply of the protein, which is essential for experimental reproducibility and potential clinical use.
