NRG1 Human
(a) Analysis by RP-HPLC.
(b) Analysis by SDS-PAGE.
Description
Recombinant Human Neuregulin-1 beta 2 produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 61 amino acids and having a total molecular mass of 7.0kDa. NRG-1 is purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
Heregulin-B2, also known as Neuregulin-1 (NRG1), is a member of the neuregulin family of proteins. These proteins play a crucial role in cell signaling, particularly in the development and function of the nervous system and heart. Heregulin-B2 is a signaling protein that interacts with the ErbB family of receptors, specifically ErbB2 and ErbB4, to mediate various cellular processes .
Heregulin-B2 is a recombinant protein produced in Escherichia coli (E. coli). It is a single, non-glycosylated polypeptide chain containing 61 amino acids, with a molecular mass of approximately 7055 Daltons . The protein is typically lyophilized (freeze-dried) and can be reconstituted for use in various experimental applications.
Heregulin-B2 plays a significant role in the development and maintenance of the nervous system and cardiac muscle cells. It induces the phosphorylation of ErbB2/ErbB4 receptor heterodimers, which leads to cardiomyocyte differentiation and improved heart structure and function . Additionally, Heregulin-B2 has been shown to stimulate the proliferation of human MCF-7 cells, a breast cancer cell line, under serum-free conditions .
The interaction of Heregulin-B2 with ErbB receptors triggers a cascade of intracellular signaling pathways. These pathways involve the activation of various kinases and scaffold proteins, leading to changes in cell morphology, migration, and proliferation . In breast cancer cells, Heregulin-B2 signaling has been implicated in the development of an aggressive phenotype and resistance to anti-HER2 therapies such as trastuzumab and trastuzumab-emtansine .
Heregulin-B2’s role in cell signaling and proliferation makes it a potential target for therapeutic interventions in diseases such as cancer and heart disease. Research has shown that Heregulin-B2 can reduce the damage to myocardial cells caused by ischemia, hypoxia, and viral infections . In breast cancer, understanding the molecular mechanisms of Heregulin-B2 signaling can help develop more effective treatments to control cell motility and drug resistance .
Lyophilized Heregulin-B2 is stable at room temperature for up to three weeks but should be stored desiccated below -18°C for long-term storage. Upon reconstitution, it should be stored at 4°C for short-term use (2-7 days) and below -18°C for future use. It is important to avoid repeated freeze-thaw cycles to maintain the protein’s activity .
