NNMT Mouse
Nicotinamide N-methyltransferase , Nnmt, icotinamide N-methyltransferase isoform1.
Greater than 90.0% as determined by SDS-PAGE.
Description
NNMT Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 288 amino acids (1-264a.a) and having a molecular mass of 32.1kDa.
NNMT is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Nicotinamide N-methyltransferase (NNMT) is an enzyme that plays a crucial role in the metabolism of nicotinamide, a form of vitamin B3. It belongs to a group of enzymes known as transferases, specifically those that transfer a single carbon unit called a methyl group. NNMT is primarily found in the liver, with lower levels detected in organs like the kidneys, lungs, muscles, placenta, and heart. Its primary function is to facilitate the addition of a methyl group to nicotinamide and similar molecules, creating positively charged molecules called pyridinium ions. This process is vital for the body's detoxification system, helping to eliminate drugs and foreign compounds. NNMT utilizes a molecule called S-adenosyl methionine as the source of the methyl group during this transfer. Studies have shown a connection between NNMT levels and the severity and progression of liver cancer. This correlation makes it a potential marker for the diagnosis and monitoring of this type of cancer. Furthermore, NNMT levels in the blood have shown promise in the early identification and effective management of patients diagnosed with colorectal cancer.
This product consists of recombinant NNMT protein derived from mice. Produced in E. coli bacteria, the protein is purified and exists in a single-chain format without any sugar modifications. It encompasses 288 amino acids, including a 24-amino acid His-tag attached to its N-terminal end, and has a molecular weight of 32.1 kDa. The His-tag allows for easy purification of the protein using specialized chromatographic techniques.
The product appears as a clear, colorless liquid that has been sterilized by filtration.
The NNMT protein is supplied in a solution with a concentration of 1 mg/ml. The solution contains 20mM Tris-HCl buffer (pH 8.0), 10% glycerol to enhance stability, and 1mM DTT to prevent protein aggregation.
To ensure product stability, it is recommended to store the protein solution at 4°C if it will be used within 2-4 weeks. For extended storage, the solution should be kept frozen at -20°C. Adding a carrier protein like HSA or BSA (0.1%) is advised for long-term storage to further protect the protein. Repeated freezing and thawing of the protein should be avoided.
Analysis using SDS-PAGE reveals that the purity of the NNMT protein in this product is greater than 90%.
Nicotinamide N-methyltransferase , Nnmt, icotinamide N-methyltransferase isoform1.
MGSSHHHHHH SSGLVPRGSH MGSHMESGFT SKDTYLSHFN PRDYLEKYYS FGSRHCAENE ILRHLLKNLF KIFCLGAVKG ELLIDIGSGP TIYQLLSACE SFTEIIVSDY TDQNLWELQK WLKKEPGAFD WSPVVTYVCD LEGNRMKGPE KEEKLRRAIK QVLKCDVTQS QPLGGVSLPP ADCLLSTLCL DAACPDLPAY RTALRNLGSL LKPGGFLVMV DALKSSYYMI GEQKFSSLPL GWETVRDAVE EAGYTIEQFE VISQNYSSTT SNNEGLFSLV GRKPGRSE.
Product Science Overview
Nicotinamide N-Methyltransferase (NNMT) is a metabolic enzyme that plays a crucial role in the methylation of nicotinamide and other pyridines, forming pyridinium ions. This enzymatic activity is significant for the biotransformation of various drugs and xenobiotic compounds. NNMT uses S-adenosyl methionine as the methyl donor in this process .
The recombinant mouse NNMT protein is typically produced in E. coli and is often tagged with a His-tag for purification purposes. The protein consists of 264 amino acids and has a predicted molecular mass of approximately 32.1 kDa . The recombinant form is used in various research applications to study its function and role in different biological processes.
NNMT is responsible for the N-methylation of nicotinamide, a form of vitamin B3, which is a crucial step in the metabolism of this compound. The enzyme’s activity impacts the methylation potential within cells, influencing DNA and histone epigenetic modifications . This makes NNMT an important player in cellular metabolism and epigenetic regulation.
NNMT has been found to be overexpressed in various solid cancer tissues and body fluids, including serum, urine, and saliva . Its overexpression is associated with increased tumorigenesis and chemoresistance. Knockdown studies of NNMT have shown a significant decrease in tumorigenesis and chemoresistance capacity, highlighting its potential as a therapeutic target .
Recombinant mouse NNMT is widely used in research to understand its role in cancer metabolism and epigenetics. It serves as a valuable tool for studying the enzyme’s function, regulation, and potential as a biomarker for cancer diagnosis and treatment . Additionally, the development of NNMT inhibitors is an active area of research, aiming to find more potent and selective inhibitors for therapeutic purposes .
