NNMT Antibody
Description
Product Specs
Product Science Overview
Nicotinamide N-Methyltransferase (NNMT) is a metabolic enzyme that plays a crucial role in the methylation process, impacting DNA and histone epigenetic modifications. NNMT is involved in the conversion of nicotinamide to 1-methylnicotinamide, a process that utilizes S-adenosylmethionine (SAM) as a methyl donor. This enzyme has garnered significant attention due to its overexpression in various solid cancer tissues and its potential as a biomarker and therapeutic target .
NNMT is a cytosolic enzyme that belongs to the family of methyltransferases. It is composed of a single polypeptide chain and has a molecular weight of approximately 29 kDa. The enzyme’s active site binds to both nicotinamide and SAM, facilitating the transfer of a methyl group from SAM to nicotinamide. This reaction produces 1-methylnicotinamide and S-adenosylhomocysteine (SAH).
NNMT is involved in several physiological processes, including the regulation of cellular methylation potential and energy metabolism. Its activity influences the levels of nicotinamide adenine dinucleotide (NAD+), a critical coenzyme in redox reactions and cellular energy production. By modulating NAD+ levels, NNMT indirectly affects various metabolic pathways and cellular functions.
NNMT overexpression has been observed in multiple cancer types, including lung, liver, and bladder cancers. Elevated NNMT levels are associated with increased tumorigenesis, chemoresistance, and poor prognosis. The enzyme’s role in cancer is linked to its ability to alter the methylation landscape of cancer cells, thereby promoting oncogenic pathways and resistance to therapy .
Mouse anti-human NNMT antibodies are monoclonal antibodies developed to specifically target and bind to human NNMT. These antibodies are used in various research and diagnostic applications, including Western blotting, immunohistochemistry, and enzyme-linked immunosorbent assays (ELISA). They are valuable tools for studying NNMT expression and function in both normal and cancerous tissues .
Given its significant role in cancer, NNMT is considered a promising target for therapeutic intervention. Inhibitors of NNMT, such as the natural compound yuanhuadine, have shown potential in reversing drug resistance in cancer cells. Ongoing research aims to develop more potent and selective NNMT inhibitors for use in cancer therapy .
