NME4 Human, Active
Nucleoside diphosphate kinase mitochondrial, Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D.
Greater than 90.0% as determined by SDS-PAGE.
Description
NME4 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (33-187a.a.) and having a molecular mass of 19.6kDa.
NME4 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
NME4, or Non-Metastatic Cells 4, is a protein belonging to the NDK family and functions as a nucleoside diphosphate kinase within mitochondria. This enzyme plays a vital role in the synthesis of nucleoside triphosphates, excluding ATP, by facilitating the transfer of gamma-phosphates using a phosphohistidine intermediate mechanism. NME4 is encoded by the nm23 gene and is known for its widespread presence.
Recombinant human NME4, expressed in E. coli, is available as a single, non-glycosylated polypeptide chain comprising 176 amino acids (residues 33-187). With a molecular weight of 19.6 kDa, this protein variant includes a 21 amino acid His tag fused at the N-terminus. Purification is achieved through proprietary chromatographic techniques.
The provided NME4 solution has a concentration of 0.5 mg/ml and is formulated in a buffer containing 40% glycerol, 20 mM Tris-HCl (pH 8.0), and 0.2 M NaCl.
For short-term storage (2-4 weeks), the NME4 solution should be kept at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. The addition of a carrier protein such as HSA or BSA (0.1%) is advised for long-term storage. Repeated freezing and thawing of the solution should be avoided.
The purity of NME4 is determined to be greater than 90% based on SDS-PAGE analysis.
NME4 exhibits a specific activity exceeding 120 units/mg. This activity is measured as the enzyme's capacity to convert 1.0 µmole of both ATP and TDP to ADP and TTP, respectively, within one minute at a pH of 7.5 and a temperature of 25°C. The assay employs a coupled system with pyruvate kinase (PK) and lactate dehydrogenase (LDH).
Nucleoside diphosphate kinase mitochondrial, Nucleoside diphosphate kinase, mitochondrial, NDK, NDPKD, nm23-H4, NM23D.
MGSSHHHHHH SSGLVPRGSH MPSWTRERTL VAVKPDGVQR RLVGDVIQRF ERRGFTLVGM KMLQAPESVL AEHYQDLRRK PFYPALIRYM SSGPVVAMVW EGYNVVRASR AMIGHTDSAE AAPGTIRGDF SVHISRNVIH ASDSVEGAQR EIQLWFQSSE LVSWADGGQH SSIHPA
Product Science Overview
NME4 is encoded by the nm23 gene and is located in the mitochondria. The human recombinant form of NME4 is produced in Escherichia coli (E. coli) and is a single, non-glycosylated polypeptide chain containing 176 amino acids, with a molecular mass of approximately 19.6 kDa . The recombinant protein is often fused to a 21 amino acid His tag at the N-terminus to facilitate purification through chromatographic techniques .
NME4 is involved in the transfer of gamma-phosphates between nucleoside diphosphates and triphosphates, which is essential for the synthesis of nucleoside triphosphates other than ATP . This activity is crucial for maintaining the balance of nucleotides within the cell, which is necessary for various cellular processes, including DNA and RNA synthesis .
The specific activity of NME4 is defined as the amount of enzyme that converts 1.0 micromole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25°C in a coupled system with pyruvate kinase (PK) and lactate dehydrogenase (LDH) . This high level of activity underscores the enzyme’s importance in cellular metabolism.
The recombinant NME4 protein is typically formulated in a solution containing 40% glycerol, 20 mM Tris-HCl buffer (pH 8.0), and 0.2 M NaCl . For short-term storage, it is recommended to keep the protein at 4°C if it will be used within 2-4 weeks. For long-term storage, the protein should be frozen at -20°C, and it is advisable to add a carrier protein, such as 0.1% human serum albumin (HSA) or bovine serum albumin (BSA), to prevent degradation . Multiple freeze-thaw cycles should be avoided to maintain the protein’s integrity .
NME4 has been studied extensively for its role in cellular metabolism and its potential implications in various diseases, including cancer. The downregulation of NME proteins has been associated with increased metastatic potential in certain cancer cell lines, highlighting their importance in maintaining cellular homeostasis and preventing metastasis .
Research into NME4 and other members of the NME family continues to provide valuable insights into their functions and potential therapeutic applications. Understanding the mechanisms by which these proteins regulate nucleotide synthesis and cellular metabolism could lead to the development of novel treatments for cancer and other diseases .
In summary, Non-Metastatic Cells 4 (Human Recombinant, BioActive) is a vital enzyme involved in nucleotide synthesis and cellular metabolism. Its recombinant form, produced in E. coli, is widely used in research to study its functions and potential therapeutic applications.
