NME3 Human
Description
NME3 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
Product Science Overview
NME3 is a single, non-glycosylated polypeptide chain consisting of 169 amino acids, with a molecular mass of approximately 19.1 kDa . The recombinant form of NME3 is typically produced in Escherichia coli (E. coli) and is fused to a 21 amino acid His tag at the N-terminus to facilitate purification . The protein is purified using proprietary chromatographic techniques to ensure high purity, often greater than 95% as determined by SDS-PAGE .
NME3 is involved in several critical biological functions:
- Nucleoside Triphosphate Synthesis: NME3 is essential for the synthesis of nucleoside triphosphates, which are the building blocks of DNA and RNA .
- Apoptosis Induction: NME3 has a role in the induction of apoptosis, the process of programmed cell death, which is vital for maintaining cellular homeostasis and eliminating damaged or cancerous cells .
- Hematopoiesis: NME3 is expressed during the early stages of myeloid differentiation of highly purified CD34+ cells, indicating its importance in the formation of blood cells .
NME3 is considered a potential metastasis suppressor. Studies have shown that NME3 levels are lower in highly metastatic cells compared to cells with lower metastatic potential . This suggests that NME3 may play a role in inhibiting the spread of cancer cells, making it a target of interest for cancer research and potential therapeutic interventions .
The recombinant NME3 protein is typically formulated in a solution containing 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, 2mM DTT, and 50% glycerol . For short-term storage, it is recommended to keep the protein at 4°C if it will be used within 2-4 weeks. For long-term storage, the protein should be frozen at -20°C, and it is advisable to add a carrier protein (0.1% HSA or BSA) to prevent degradation .
