NME3 Human, Active
Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3.
Greater than 95.0% as determined by SDS-PAGE.
Description
NME3 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 169 amino acids (22-169 a.a.) and having a molecular mass of 19.1kDa.
NME3 is fused to a 21 amino acid His tag at N-Terminus and purified by proprietary chromatographic techniques.
Product Specs
NME3, also known as Non-Metastatic Cells 3, may play a role in suppressing metastasis. Studies have shown lower levels of NME3 in highly metastatic cells compared to cells with lower metastatic potential. This protein is involved in essential cellular processes such as nucleoside triphosphate synthesis, apoptosis induction, and hematopoiesis. NME3 expression is observed during the early stages of myeloid differentiation in highly purified CD34+ cells.
Recombinant human NME3, expressed in E. coli, is a single, non-glycosylated polypeptide chain. This protein consists of 169 amino acids (with amino acids 22-169 present) and has a molecular weight of 19.1 kDa. A 21 amino acid His tag is fused to the N-terminus of NME3. Purification is achieved using proprietary chromatographic techniques.
The NME3 solution is provided at a concentration of 0.5 mg/ml. The solution is composed of 50% glycerol, 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 2mM DTT.
For short-term storage (2-4 weeks), the NME3 solution can be stored at 4°C. For extended storage, it is recommended to freeze the solution at -20°C. Adding a carrier protein (0.1% HSA or BSA) is advisable for long-term storage. To maintain the integrity of the protein, avoid repeated cycles of freezing and thawing.
Analysis by SDS-PAGE has determined the purity to be greater than 95.0%.
The specific activity is measured as the amount of enzyme required to convert 1.0 micromole of ATP and TDP to ADP and TTP per minute. This reaction occurs at pH 7.5 and 25°C in a coupled system with pyruvate kinase (PK) and lactate dehydrogenase (LDH). NME3 exhibits a specific activity greater than 150 units/mg.
Nucleoside diphosphate kinase 3, c371H6.2, DR-nm23, KIAA0516, NDPK-C, NDPKC, NM23-H3, NM23H3.
MGSSHHHHHH SSGLVPRGSH MERTFLAVKP DGVQRRLVGE IVRRFERKGF KLVALKLVQA SEELLREHYA ELRERPFYGR LVKYMASGPV VAMVWQGLDV VRTSRALIGA TNPADAPPGT IRGDFCIEVG KNLIHGSDSV ESARREIALW FRADELLCWE DSAGHWLYE
Product Science Overview
NME3 Human Recombinant is produced in E. coli and is a single, non-glycosylated polypeptide chain containing 169 amino acids (22-169 a.a.) with a molecular mass of 19.1 kDa . The recombinant protein is fused to a 21 amino acid His tag at the N-terminus and is purified using proprietary chromatographic techniques . The formulation typically includes 50% glycerol, 20 mM Tris-HCl buffer (pH 8.0), 0.1 M NaCl, and 2 mM DTT .
NME3 is essential for the synthesis of nucleoside triphosphates, which are vital for various cellular functions . The specific activity of NME3 is defined as the amount of enzyme that converts 1.0 µmole each of ATP and TDP to ADP and TTP per minute at pH 7.5 at 25°C in a coupled system with PK/LDH . This activity is greater than 150 units/mg .
NME3 is involved in several critical cellular processes:
- Metastasis Suppression: NME3 is a possible suppressor of metastasis, with lower levels observed in highly metastatic cells .
- Apoptosis Induction: NME3 plays a role in the induction of apoptosis, a process of programmed cell death that is essential for maintaining cellular homeostasis .
- Hematopoiesis: NME3 is crucial for hematopoiesis, the process of forming new blood cells .
