NGB Human, His
Greater than 90% as determined by SDS-PAGE.
Description
Neuroglobin Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 161 amino acids (1-151a.a) and having a molecular mass of 18kDa. NGB is fused to 10 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques.
Product Specs
MKHHHHHHAS MERPEPELIR QSWRAVSRSP LEHGTVLFAR LFALEPDLLP LFQYNCRQFS SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLA SLGRKHRAVG VKLSSFSTVG ESLLYMLEKC LGPAFTPATR AAWSQLYGAV VQAMSRGWDG E.
Product Science Overview
Neuroglobin is a single, non-glycosylated polypeptide chain with a molecular mass of approximately 18 kDa . The recombinant form of neuroglobin, tagged with a His (histidine) tag, is produced in Escherichia coli (E. coli) expression systems . The His tag, typically consisting of six histidine residues, is fused to the N-terminus of the protein, facilitating its purification through affinity chromatography .
Neuroglobin is believed to function as an oxidative stress-responsive sensor in the brain, playing a role in signal transduction . Its expression is upregulated in response to neuronal hypoxia and focal cerebral ischemia, suggesting its involvement in neuroprotection . Experimental studies have shown that inhibiting neuroglobin expression reduces neuronal survival after hypoxia, while overexpression enhances it .
The recombinant neuroglobin is typically provided as a lyophilized (freeze-dried) powder, which should be reconstituted in deionized water to prepare a working stock solution . It is recommended to store the lyophilized protein at -20°C and avoid repeated freezing and thawing cycles to maintain its stability .
